Experimental Science
Expression and significance of nuclear factor-κB-related proteins in idiopathic orbital inflammatory pseudotumor
Linqi Yang, Pengxiang Zhao, Yanan Wu, Xujuan Zhang, Lei Shang, Mengyu Liu, Xiao Liu, Jianmin Ma, Xuemei Ma
Published 2017-09-10
Cite as Chin J Exp Ophthalmol, 2017, 35(9): 786-791. DOI: 10.3760/cma.j.issn.2095-0160.2017.09.004
Abstract
BackgroundIdiopathic orbital inflammatory pseudotumor (IOIP) is a commom orbital disease, with serious eye symptoms and replase tendency, and its pathogenesis is still unclear.Nuclear factor-κB (NF-κB)-related proteins participate in many important pathophysiological process, however, whether NF-κB plays a role in the IOIP process is worthy of attention.
ObjectiveThis study was to explore the roles of NF-κB pathway in IOIP pathogenesis.
MethodsTwenty-four IOIP specimens were collected during surgery in Beijing Tongren Hospital from September 2010 to May 2016.The histopathological characteristics of IOIP were examined by hematoxylin and eosin staining.The expression and location of NF-κB/p65, p-p65, p50 and inhibitor of κB (IκB-α) were detected by immunohistochemistry and verified by immunocytochemistry and Western blot assay.
ResultsThe histopathological features of IOIP were numerous small lymphocyte infiltraion and fibrous tissue proliferation, and a lot of epithelioid cells were seen in lacrimal gland-involved specimens.NF-κB/p65 was positively expressed in the cytoplasm of all 24 specimens and the nucleus in 15 specimens with the expressing rate of 62.5%.p50 was expressed in the cytoplasm in 22 specimens with the expressing rate of 91.7% and in the nucleus in 17 specimens with the expressing rate of 70.8%.The positive expression of p-p65 was found in 22 specimens with the expressing rate of 91.7%, and IκB-α was expressed in the cytoplasm of 11 specimens with the expressing rate of 45.8%.These results were confirmed by immunocytochemistry and Western blot assay.
ConclusionsNF-κB pathway is activiated during IOIP process, and NF-κB pathway may be involved in the pathogenesis of IOIP.
Key words:
Nuclear factor-kappa B/metabolism; Orbital inflammatory pseudotumor/pathology; Inflammation; Immunochemistry; Humans; Nuclear proteins/metabolism; Signal transduction pathway
Contributor Information
Linqi Yang
College of Life Science and Bioengineering of Beijing University of Technology, Beijing 100124, China
Pengxiang Zhao
College of Life Science and Bioengineering of Beijing University of Technology, Beijing 100124, China
Yanan Wu
College of Life Science and Bioengineering of Beijing University of Technology, Beijing 100124, China
Xujuan Zhang
College of Life Science and Bioengineering of Beijing University of Technology, Beijing 100124, China
Lei Shang
College of Life Science and Bioengineering of Beijing University of Technology, Beijing 100124, China
Mengyu Liu
College of Life Science and Bioengineering of Beijing University of Technology, Beijing 100124, China
Xiao Liu
Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Key Laboratory of Ophthalmology and Visual Science, Beijing 100730, China
Jianmin Ma
Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Key Laboratory of Ophthalmology and Visual Science, Beijing 100730, China
Xuemei Ma
College of Life Science and Bioengineering of Beijing University of Technology, Beijing 100124, China
