To observe anatomy of liver and establish 70% and 90% standard partial hepatectomy model in C57BL/6J mice.

Sixty adult mice with a C57BL/6J SPF stage were randomly equably divided into three groups, group A, group B and group C. Weight of body, total liver, and every single liver lobe were weighed in group A, ratio of total liver weight/body weight, and proportion of 70% and 90% liver was calculated, respectively. 70% partial hepatectomy in group B and 90% partial hepatectomy in group C were performed, using step by step visually ligaturing anatomic functional unit of liver by only threads.

The weight of total liver, body, left lateral lobe, median lobe, right anterior lobe, right posterior lobe and caudate lobe in mice were (1.325±0.029) g, (26.500±1.140) g, (0.433±0.041) g, (0.411±0.035) g, (0.140±0.011) g, (0.175±0.015) g, (0.099±0.006) g, respectively, the ratio of total liver weight/body weight was (4.92±0.13)%, the weight of left lateral lobe and median lobe accounted for 70% of the whole liver, and the weight of other parts except caudate lobe preserved was 90%. The successful operation rate in both group B and C was 100%. The survival rate was 100% one week after operation in group B, 30% (6/20) one day after operation in group C, respectively, and no survival in group C two days after operation.

This study ascertains the proportion of every liver lobe in mice for the first time. Using step by step visually ligaturing anatomic functional unit of liver by only threads, it is easy to establish stable and credible 70% and 90% partial hepatectomy model in mice.

Partial hepatectomy; Application anatomy; Mice; Model; Standard