Basic Researches
Effect and mechanism of STEAP3 on inhibiting the proliferation ability of hepatocellular carcinoma cells
Weiming Fan, Zhicheng Yao, Jianliang Xu, Zhiyong Xiong, Ruixi Li, Boxuan Zhou, Meihai Deng
Published 2017-04-10
Cite as Chin J Hepat Surg(Electronic Edition), 2017, 06(2): 134-138. DOI: 10.3877/cma.j.issn.2095-3232.2017.02.014
Abstract
ObjectiveTo investigate the effect and mechanism of six transmembrane epithelial antigen prostate 3 (STEAP3) on inhibiting the proliferation of hepatocellular carcinoma (HCC) cells.
MethodsHCC cell lines 7721-shSTEAP3 and QGY-shSTEAP3 expressing STEAP3 were stably interfered in the interference group. And the control cells 7721-VECTOR and QGY-VECTOR were also established in the control group. The expression of STEAP3 mRNA was detected by RT-PCR. The proliferation ability of the cells was detected by CCK-8 assay. Cell cycle was detected by flow cytometry. The expression of cell cycle-associated proteins were detected by Western blot. The experimental data were compared using t test.
ResultsHCC cell lines expressing STEAP3 were stably interfered successfully. The expression levels of STEAP3 mRNA in the 7721-shSTEAP3 and QGY-shSTEAP3 cells of the interference group were respectively (5.10±0.70)×10-3 and (0.09±0.02)×10-3, significantly lower than (7.80±0.07)×10-3 and (7.00±0.21)×10-3 in the 7721-VECTOR and QGY-VECTOR cells of the control group (t=-9.60, -57.16; P<0.05). Cell growth curve showed that the proliferation ability of 7721-shSTEAP3 and QGY-shSTEAP3 cells in the interference group were significantly enhanced compared with that of 7721-VECTOR and QGY-VECTOR cells in the control group. Cell cycle detection showed that (55.23±0.51)% and (57.38±0.78)% of 7721-shSTEAP3 and QGY-shSTEAP3 cells in the interference group were in G0/G1 phase, significantly lower than (68.25±0.28)% and (77.55±0.89)% of 7721-VECTOR and QGY-VECTOR cells in the control group (t= -7.83, -27.39; P<0.05). The expression levels of cyclin D1 and protein cyclin-dependent kinase 4 (CDK4) of the 7721-shSTEAP3 and QGY-shSTEAP3 cells in the interference group were respectively 0.730±0.017, 1.090±0.028 and 0.780±0.063, 1.310±0.031, significantly higher than 0.660±0.059, 0.780±0.085 and 0.530±0.014, 0.920±0.024 of the 7721-VECTOR and QGY-VECTOR cells in the control group (t=4.47, 6.87 and 12.33, 4.89; P<0.05).
ConclusionsSTEAP3 exerts an effect on inhibiting the proliferation ability of HCC cells. The mechanism may probably be correlated with blocking the G1 phase of cell cycle.
Key words:
Proteins; Liver neoplasms; Cell proliferation; Cell cycle
Contributor Information
Weiming Fan
Department of Hepatobiliary Surgery, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China
Zhicheng Yao
Department of General Surgery, Lingnan Hospital, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510530, China
Jianliang Xu
Department of Hepatobiliary Surgery, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China
Zhiyong Xiong
Department of General Surgery, Lingnan Hospital, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510530, China
Ruixi Li
Department of Hepatobiliary Surgery, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China
Boxuan Zhou
Department of Hepatobiliary Surgery, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China
Meihai Deng
Department of Hepatobiliary Surgery, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China
