Original Article
Influence of aluminum chloride exposure on embryonic development of zebrafish and neurobehavior of juvenile fish
Chen Jianping, Shang Nan, He Kaihong, Zhang Ling, Niu Qiao, Zhang Qinli
Published 2017-03-20
Cite as Chin Ind Hyg Occup Dis, 2017, 35(3): 166-170. DOI: 10.3760/cma.j.issn.1001-9391.2017.03.002
Abstract
ObjectiveTo investigate the influence of aluminum chloride (AlCl3) solution on the embryon-ic development of zebrafish and neurobehavior of juvenile fish.
MethodsThe embryos of zebrafishat 6 hours after fertilization were exposed to AlCl3 solution at a concentration of 0, 55.0, 60.5, 66.6, 73.5, 80.5, or 100.0 mg/L, and embryonic hatching rates at 48 and 72 hours after fertilization were calculated. The embryos of zebrafishat 6 hours after fertilization were exposed to AlCl3 solution at a concentration of 0, 60.0, 72.0, 86.4, 103.7, or 124.4 mg/L, and the embryonic mortality rates at 12, 24, 48, 72, and 96 hours after fertilization were calculat-ed. The embryos of zebrafish at 6 hours after fertilization were exposed to AlCl3 solution at a concentration of 0, 50, 100, 200, 400, or 800 μg/L, and the changes in the neurobehavior of juvenile fish were observed after hatching, including touch-escape reaction at 72 hours after fertilization and autonomic movement and panic es-cape reflex at 7 days after fertilization.
ResultsCompared with the 0 mg/L group, the≥66.6 mg/L group had a sig-nificant reduction in embryonic hatching rate at 48 and 72 hours after fertilization, and the ≥72.0 mg/L group had a significant increase in embryonic mortality rate at 96 hours after fertilization (P<0.05) . Compared with the 0 μg/L group, the≥100 μg/L group had a significant reduction in the number of times of touch-escape reaction (P<0.05) .Compared with the 0 and 50 μg/L groups, the 100-800 μg/L groups had significant reductions in total movement distance and average speed (P<0.05) . Compared with the dark period before illumination, all groups had a significant increase in movement speed during the light period of the panic escape reflex test (i.e., the third minute) (P<0.05) ; within 2 minutes after the light was turned off, there was no significant change in movement speed in the 0-200 μg/L groups (P>0.05) ; the 400 and 800 μg/L groups had a significant increase in movement speed (P<0.05) .
ConclusionAlCl3 exposure may cause embryonic developmental disorder in zebrafish and ab-normal neurobehavior in juvenile fish.
Key words:
Aluminium chloride; Zebrafish; Embryos; Developmental neurotoxicity
Contributor Information
Chen Jianping
Department of Occupational Medicine, Shanxi Medical University, Taiyuan 030001, China
Shang Nan
He Kaihong
Zhang Ling
Niu Qiao
Zhang Qinli
