Basic Science Investigations
Diagnostic value of 18F-FDG micro-PET/CT in rat models of intracerebral hemorrhage in vivo
Yang Fanhui, Yang Chaoxian, Zhang Chunyin
Published 2021-04-25
Cite as Int J Radiat Med Nucl Med, 2021, 45(4): 231-236. DOI: 10.3760/cma.j.cn121381-202003023-00045
Abstract
ObjectiveTo assess the diagnostic value of 18F-fluorodeoxyglucose (FDG) micro-PET/CT in evaluating an intracerebral hemorrhage (ICH) model in rats in vivo.
MethodsA simple random sampling method was used to select 32 healthy male adult SD rats, 4 of which were placed in the sham operation group and 28 rats were injected with 0.125 U/μL collagenase Ⅳ into the right basal ganglia to induce ICH (ICH model group). In the sham operation group, 0.9% saline was used instead of collagenase Ⅳ to make the sham model. The ICH model group was divided into seven groups by simple random sampling according to the time after ICH, which were 6, 24, 48 h and 3, 5, 7, 14 d (4 rats in each group). The sham operation and seven ICH model groups (6, 24, 48 h and 3, 5, 7, 14 d) underwent the neurological impairment scoring and18F-FDG micro-PET/CT imaging. The hematoma volume at each time point in the ICH model group was calculated according to 18F-FDG micro-PET/CT imaging to delineate the region of interest (ROI) and Tada's formula. After imaging, the head was decapitated and the brain was obtained for hematoma observation and histopathological examination. The hematoma volume obtained by the two methods at the same time was compared by pairedt-test and evaluated by Pearson correlation analysis.
ResultsIn the sham operation group: the neurological impairment scores were zero; and micro-PET/CT clearly showed homogeneous 18F-FDG uptake in the brain tissue. The ICH model group: the neurological impairment scores were (2.21±0.30), (3.51±0.66), (2.83±0.20), (2.12±0.50), (1.44±0.37), (1.02±0.25) and (0.51±0.12) at 6, 24, 48 h and 3, 5, 7, 14 d after ICH, respectively. At each time point, the 18F-FDG uptake decreased or became defective in the right basal ganglia of the rat brain. The cerebral hematoma volumes evaluated by 18F-FDG micro-PET/CT were (24.05±3.00), (27.19±1.25), (25.58±1.57), (21.94±0.98), (19.88±1.53), (18.35± 2.11) and (16.29±1.53) mm3, respectively. The cerebral hematoma volumes evaluated by Tada's formula were (23.17±1.93), (26.09±1.35), (24.64±1.95), (21.31±1.32), (19.07±1.64), (17.29±1.38), and (15.63±1.98) mm3, respectively. No significant difference in the hematoma volume was found between two methods at each time point in the ICH model group (t=1.18-3.06, all P>0.05). The cerebral hematoma volume obtained by the two methods was significantly positively correlated (r=0.99, P<0.001). After the dissection of the ICH model group, irregular hematoma formation was seen in the right basal ganglia of the brain tissue, which was opposite to the radioactive sparse and defective areas shown by18F-FDG micro-PET/CT.
Conclusion18F-FDG micro-PET/CT can accurately display the position, shape and size of the hematoma after ICH, and thus can be to verify the success of the rat ICH model in vivo.
Key words:
Intracerebral hemorrhage; Positron-emission tomography; Tomography, X-ray computed; Fluorodeoxyglucose F18; Rats; Models, animal
Contributor Information
Yang Fanhui
Department of Nuclear Medicine, the Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, China
Nuclear Medicine and Molecular Imaging Key Laboratory of Sichuan Province, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, China
Yang Chaoxian
Department of Neurobiology, Southwest Medical University, Luzhou 646000, China
Zhang Chunyin
Department of Nuclear Medicine, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, China
