Pain Management and Research
Relationship between IL-17 and PI3K/Akt signaling pathway in dorsal root ganglion of rats with bone cancer pain
Li Hongbo, Wang Yungong, Yao Ming, Xu Longsheng, Wang Lina, Zheng Ying, Ni Huadong, Li Jun, Lian Qingquan
Published 2016-07-20
Cite as Chin J Anesthesiol, 2016, 36(7): 827-830. DOI: 10.3760/cma.j.issn.0254-1416.2016.07.014
Abstract
ObjectiveTo investigate the relationship between interleukin-17 (IL-17) and phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway in the dorsal root ganglion (DRG) of rats with bone cancer pain (BCP).
MethodsForty-four pathogen-free adult female unmated Sprague-Dawley rats, aged 9 weeks, weighing 180-200 g, were randomly divided into 4 groups (n=11 each) using a random number table: sham operation group (group S); BCP group (group B); BCP + IL-17 antibody group (group BI); BCP + phosphate buffer solution (the solvent) group (group BP). BCP was induced by injecting Walker256 mammary gland cancer cell suspension 10 μl into the medullary cavity of the left tibia in B, BI and BP groups, while group S received intra-tibial inoculation of 10 μl Hank′s solution.At 9-11 days after BCP, PBS 20 μl/d and IL-17 antibody (1 mg/ml) 20 μl/d were injected intrathecally once a day in BP and BI groups, respectively.Before BCP (T 0), at 5 days after BCP (T1), before administration on day 9 after BCP (T2) and at 30 min after administration on day 11 after BCP (T3), the mechanical pain threshold was measured.After measurement of the pain threshold on day 11 after BCP, the animals were sacrificed, and the DRGs of the lumbar segment (L4-6) were removed for determination of the expression of PI3K, phosphorylated Akt (p-Akt) and Akt by Western blot.
ResultsCompared with group S, the mechanical pain threshold was significantly decreased at T1-3, and the expression of PI3K and p-Akt in DRGs was significantly up-regulated in B, BI and BP groups (P<0.01=. Compared with group B, the mechanical pain threshold was significantly increased at T3, the expression of PI3K and p-Akt in DRGs was significantly down-regulated (P<0.01=, and no significant change was found in the mechanical pain threshold at T1, 2 in group BI, and no significant change was found in the parameters mentioned above in group BP (P>0.05). There was no significant difference in the expression of Akt in DRGs among the four groups (P>0.05).
ConclusionIL-17 in the DRG is involved in the maintenance of BCP probably through activating PI3K/Akt signaling pathway in rats.
Key words:
Bone neoplasms; Pain; Interleukin-17; 1-Phosphatidylinositol 3-kinase; Protein-serine-threonine kinases
Contributor Information
Li Hongbo
Second Clinical Medical College, Wenzhou Medical University, Wenzhou 325027, China
Wang Yungong
Second Clinical Medical College, Wenzhou Medical University, Wenzhou 325027, China
Yao Ming
Second Clinical Medical College, Wenzhou Medical University, Wenzhou 325027, China
Xu Longsheng
Anesthesia and Pain Medicine Center, First Affiliated Hospital of Jiaxing University, Jiaxing 314000, China
Wang Lina
Department of Anesthesia, First Affiliated Hospital of Soochow University
Department of Anesthesia, First Affiliated Hospital of Soochow University, Suzhou 215006, China
Zheng Ying
Anesthesia and Pain Medicine Center, First Affiliated Hospital of Jiaxing University, Jiaxing 314000, China
Ni Huadong
Anesthesia and Pain Medicine Center, First Affiliated Hospital of Jiaxing University, Jiaxing 314000, China
Li Jun
Second Clinical Medical College, Wenzhou Medical University, Wenzhou 325027, China
Lian Qingquan
Second Clinical Medical College, Wenzhou Medical University, Wenzhou 325027, China
