Critical Care Medicine
Effect of electroacupuncture postconditioning on expression of microRNA-1 in rats with arrhythmia induced by myocardial ischemia-reperfusion
Liu Yanqiu, Tang Jian, Fu Xiaokui, Huang Suisui, Ren Wenxin, Gao Hong
Published 2020-02-20
Cite as Chin J Anesthesiol, 2020, 40(2): 246-249. DOI: 10.3760/cma.j.issn.0254-1416.2020.02.030
Abstract
ObjectiveTo evaluate the effect of electroacupuncture postconditioning on the expression of microRNA-1 (miRNA-1) in rats with arrhythmias induced by myocardial ischemia-reperfusion (I/R).
MethodsTwenty-four clean-grade healthy adult male Sprague-Dawley rats, aged 2-3 months, weighing 280-320 g, were divided into 3 groups (n=8 each) using a random number table method: sham operation group (S group), I/R group and electroacupuncture postconditioning group (EP group). The model of myocardial reperfusion arrhythmia was established by ligating the left anterior descending branch of coronary artery for 30 min followed by 30-min reperfusion in anesthetized rats.The artery was only isolated without ligation after thoracotomy in group S. Bilateral Neiguan acupoints were stimulated for 30 min starting from the time point immediately after reperfusion in EP group.The occurrence of arrhythmia during reperfusion was recorded.The rats were sacrificed at 30 min of reperfusion, and hearts were removed for examination of the pathological changes of the myocardium and for determination of miRNA-1 expression (by quantitative real-time polymerase chain reaction) and expression of Kir2.1 and connexin 43 (Cx43) (by Western blot).
ResultsCompared with S group, the incidence of arrhythmia was significantly increased, the expression of miRNA-1 was up-regulated, and the expression of Kir2.1 and Cx43 was down-regulated in I/R and EP groups (P<0.05). Compared with I/R group, the incidence of arrhythmia was significantly decreased, the expression of miRNA-1 was down-regulated, and the expression of Kir2.1 and Cx43 was up-regulated in EP group (P<0.05). The pathological changes of myocardium were accentuated in I/R and EP groups when compared with S group, and the pathological changes were significantly attenuated in EP group when compared with I/R group.
ConclusionThe mechanism by which electroacupuncture postconditioning reduces the occurrence of reperfusion arrhythmia is related to up-regulation of Kir2.1 and Cx43 expression after down-regulation of miRNA-1 expression in rats.
Key words:
Electroacupuncture; Ischemic postconditioning; Myocardial reperfusion injury; Arrhythmias, cardiac; MicroRNAs
Contributor Information
Liu Yanqiu
Department of Anesthesiology, the Fourth People′s Hospital of Guiyang, Guiyang 550001, China
Tang Jian
School of Anesthesioloy, Guizhou Medical University, Guiyang 550001, China
Fu Xiaokui
School of Anesthesioloy, Guizhou Medical University, Guiyang 550001, China
Huang Suisui
School of Anesthesioloy, Guizhou Medical University, Guiyang 550001, China
Ren Wenxin
School of Anesthesioloy, Guizhou Medical University, Guiyang 550001, China
Gao Hong
the Third Affiliated Hospital of Guizhou Medical University, Duyun 558000, China
