Perioperative Complication
Relationship between dexmedetomidine-induced reduction of sevoflurane-induced neurotoxicity and NKCC1/KCC2 in neonatal rats
Li Tang, Wang Haiyun, Bu Xinyue, Yang Chenyi
Published 2021-04-20
Cite as Chin J Anesthesiol, 2021, 41(4): 445-449. DOI: 10.3760/cma.j.cn131073.20201219.00413
Abstract
ObjectiveTo evaluate the relationship between dexmedetomidine-induced reduction of sevoflurane-induced neurotoxicity and cation-chloride cotransporters Na+ -K+ -2Cl--1 (NKCC1) /K+ -2Cl--2 (KCC2) in neonatal rats.
MethodsEighty healthy male Sprague-Dawley rats at postnatal day 7 were divided into 4 groups (n=20 each) using a random number table method: control group (group C), sevoflurane anesthesia group (group S), dexmedetomidine group (group D), and sevoflurane and dexmedetomidine group (group SD). Rats were exposed to 2.5% sevoflurane for 6 h to establish the model of sevoflurane anesthesia in group S. Dexmedetomidine 1.0 μg/kg was intraperitoneally injected, and then sevoflurane anesthesia was performed in group SD.The expression of cleaved caspase-3, NKCC1 and KCC2 was detected by Western blot at 24 h after the end of anesthesia.At 35 days after the end of anesthesia, the cognitive function was assessed using the Y maze test, and the neurons in the hippocampal CA1 area were counted using the Nissan staining method.
ResultsCompared with group C, the percentage of time spent in novel arm and the number of neurons in hippocampal CA1 area were significantly decreased, the expression of cleaved caspase-3 and NKCC1 was up-regulated, the expression of KCC2 was down-regulated, and the ratio of NKCC1/KCC2 was increased in S and SD groups (P<0.05), and no change was found in the above indicators in group D (P>0.05). Compared with group S, the percentage of time spent in novel arm and the number of neurons in hippocampal CA1 area were significantly increased, the expression of cleaved caspase-3 and NKCC1 was down-regulated, the expression of KCC2 was up-regulated, and the ratio of NKCC1/KCC2 was decreased in group SD (P<0.05).
ConclusionThe mechanism of dexmedetomidine attenuating sevoflurane-induced neurotoxicity may be related to maintaining the relatively stable expression of NKCC1/KCC2 in neonatal rats.
Key words:
Dexmedetomidine; Anesthetics, inhalation; Symporters
Contributor Information
Li Tang
Department of Anesthesiology, Affiliated Hospital of Jiaxing University, The First Hospital of Jiaxing, Jiaxing 314001, China
Wang Haiyun
Department of Anesthesiology, The Third Central Hospital of Tianjin, Nankai University Affinity the Third Central Hospital The Third Central Clinical College of Tianjin Medical University Tianjin Key Laboratory of Extracorporeal Life Support for Critical Diseases Artificial Cell Engineering Technology Research Center Tianjin Institute of Hepatobiliary Disease, Tianjin 300170, China
Bu Xinyue
Department of Anesthesiology, The Third Central Hospital of Tianjin, Nankai University Affinity the Third Central Hospital The Third Central Clinical College of Tianjin Medical University Tianjin Key Laboratory of Extracorporeal Life Support for Critical Diseases Artificial Cell Engineering Technology Research Center Tianjin Institute of Hepatobiliary Disease, Tianjin 300170, China
Yang Chenyi
Department of Anesthesiology, The Third Central Hospital of Tianjin, Nankai University Affinity the Third Central Hospital The Third Central Clinical College of Tianjin Medical University Tianjin Key Laboratory of Extracorporeal Life Support for Critical Diseases Artificial Cell Engineering Technology Research Center Tianjin Institute of Hepatobiliary Disease, Tianjin 300170, China
