Perioperative Complication
Effect of electrical stimulation on lipopolysaccharide-induced activation of M1 microglia
Liu Wenjie, Wang Ye, Dong Rui, Wang Mingshan, Zhang Gaofeng, Shan Kaiyue, Chen Huai-long
Published 2022-03-20
Cite as Chin J Anesthesiol, 2022, 42(3): 265-268. DOI: 10.3760/cma.j.cn131073.20211021.00303
Abstract
ObjectiveTo evaluate the effect of electrical stimulation on lipopolysaccharide (LPS)-induced activation of M1 microglia.
MethodsThe well-growing BV2 microglia cells were divided into 3 groups (n=18 each) using a random number table method: control group (group C), group LPS, LPS and electrical stimulation group (group LE). The cells were cultured for 24 h in normal culture atmosphere in group C. In group LPS and group LE, the LPS medium culture 100 ng/ml was added, and the cells were cultured for 24 h. In group LE, cells were stimulated with 100 mV/mm direct current for 4 h before LPS incubation.The levels of tumor necrosis factor-α (TNF-α) and leukocyte interleukin-1β (IL-1β) were determined by enzyme-linked immunosorbent assay.The expression of the M1 microglia surface markers CD32 and inducible nitric oxide synase (iNOS) was detected using immunofluorescent staining.The expression of CD32 and iNOS mRNA was detected using quantitative real-time polymerase chain reaction.
ResultsCompared with group C, the concentrations of TNF-α and IL-1β were significantly increased, and the expression of CD32 and iNOS protein and mRNA was up-regulated in LPS and LE groups (P<0.05). Compared with group LPS, the concentrations of TNF-α and IL-1β were significantly decreased, and the expression of CD32 and iNOS protein and mRNA was down-regulated in group LE (P<0.05).
ConclusionsElectrical stimulation can inhibit LPS-induced activation of M1 microglia and thus alleviate the inflammatory responses.
Key words:
Electrical stimulation; Lipopolysaccharides; Microglia
Contributor Information
Liu Wenjie
Department of Anesthesiology, Qingdao Municipal Hospital Affiliated to Qingdao University, Qingdao 266071, China
Graduate School of Dalian Medical University, Dalian 116044, China
Wang Ye
Department of Anesthesiology, Qingdao Municipal Hospital Affiliated to Qingdao University, Qingdao 266071, China
Dong Rui
Department of Anesthesiology, Qingdao Municipal Hospital Affiliated to Qingdao University, Qingdao 266071, China
Wang Mingshan
Department of Anesthesiology, Qingdao Municipal Hospital Affiliated to Qingdao University, Qingdao 266071, China
Zhang Gaofeng
Department of Anesthesiology, Qingdao Municipal Hospital Affiliated to Qingdao University, Qingdao 266071, China
Shan Kaiyue
Department of Burn and Plastic Surgery, Qingdao Municipal Hospital Affiliated to Qingdao University, Qingdao 266071, China
Chen Huai-long
Department of Anesthesiology, Qingdao Municipal Hospital Affiliated to Qingdao University, Qingdao 266071, China
