Experimental study of biomimetic nanoparticle probe containing hematoporphyrin monomethyl ether and its imaging and sonodynamic therapy for breast cancer
Lin Xiaohong, Li Pan, Li Qianru, Huang Ju, Jiang Qinqin, Wan Li, Tang Rui, He Tao
Abstract
ObjectiveTo develop a biomimetic nanoparticle probe of hematoporphyrin monomethyl ether (HMME) coated with breast cancer cell membrane, to observe its ability to target homologous breast cancer cells in vitro, and to investigate its effect of enhanced photoacoustic imaging and sonodynamic therapy (SDT) for breast cancer in vitro.
MethodsThe cell membrane of breast cancer 4T1 was extracted by chemical cleavage and repeated freezing and thawing. Then the HMME-coated polylactic acid-glycolic acid copolymer biomimetic nanoparticle was prepared by double emulsification and extrusion. The basic characteristics of nanoparticles were detected. The target ability of nanoparticles to homologous breast cancer cells and the enhancement of photoacoustic imaging were observed in vitro. Singlet oxygen sensor green (SOSG) was used to verify the reactive oxygen species (ROS) production of nanoparticles, and its SDT effect on breast cancer cells was evaluated by CCK8 cytotoxicity assay.
ResultsThe size of the prepared CHP-NPs was uniform, the morphology was spherical "core-shell structure" , the particle size was (275.23±8.25)nm, and the surface potential was (-18.43±0.45)mV. It was observed that CHP-NPs could target homologous 4T1 cells under laser confocal microscopy. In vitro photoacoustic imaging experiments show that the photoacoustic signal of nanoparticles increases with the increase of its concentration. According to SOSG probe detection, CHP-NPs could produce ROS under ultrasonic irradiation.When CHP-NPs was incubated with 4T1 cells alone and no ultrasonic irradiation was used, the cell survival rate was not significantly affected. When the concentration was 0.6 mg/ml, the cell survival rate was still 95%. After ultrasonic irradiation, CCK8 experiment showed that the CHP-NPs had a significant SDT effect on breast cancer cells.
ConclusionsThe biomimetic nanomolecular probe of breast cancer cell membrane is successfully prepared. The probe has good ability to target homologous tumor, and can significantly enhance tumor photoacoustic imaging and SDT effect.
Key words:
Photoacoustic imaging; Cancer cell membrane; Hematoporphyrin monomethyl ether; Nanometer molecular probe; Sonodynamic therapy
Contributor Information
Lin Xiaohong
Department of Ultrasound, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China
Institute of Ultrasound Imaging, Chongqing Medical University, Chongqing 400010, China
Li Pan
Department of Ultrasound, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China
Institute of Ultrasound Imaging, Chongqing Medical University, Chongqing 400010, China
Li Qianru
Institute of Ultrasound Imaging, Chongqing Medical University, Chongqing 400010, China
Nuclear Medicine, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China
Huang Ju
Department of Ultrasound, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China
Institute of Ultrasound Imaging, Chongqing Medical University, Chongqing 400010, China
Jiang Qinqin
Department of Ultrasound, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China
Institute of Ultrasound Imaging, Chongqing Medical University, Chongqing 400010, China
Wan Li
Department of Ultrasound, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China
Institute of Ultrasound Imaging, Chongqing Medical University, Chongqing 400010, China
Tang Rui
Department of Ultrasound, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China
Institute of Ultrasound Imaging, Chongqing Medical University, Chongqing 400010, China
He Tao
Department of Orthopedics, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China
