：Objective To providebasis for the local immune therapy of tuberculosis by observing the absorption andsecurity of Mycobacterium vaccae (micro card) gel after administered into lung andanalysing the optimum dose and side effects. Methods Fifty Japanese long-eared rabbitswere randomly divided into five groups. Group 1 was injected with 3 ml normal sodium.Group 2 was injected with 3 ml carbomer gel. Group 3, 4, 5 were injected with 3 mlcarbomer gel with 3.75, 7.50, 9.37 mg/L Mycobacterium vaccae. The temperature, weight,blood were tested durirg four weeks. The animals were sacrificed at the end and tissuepathology was observed. Results There was no statistical significance on temperature,weight, blood among all groups. Compared with group 1, the changes of pathology in lung ofgroup 2 and group 3 were not obvious. There were no obvious changes on the bronchiolebetween group 4,5 and group 1, alveolar structure remained intact, interval mildlywidened,and there was lymphocytic infiltration in interval. Conclusions Gel withMycobacterium vaccae administered in lung has better security.