Original Article
Changes of ROS, MDA, IL-6 and TNF-α in lung tissue and skeletal muscle cell of COPD rats
He Jialin, Hu Ruicheng, Dai Aiguo
Published 2020-10-20
Cite as Int J Respir, 2020, 40(20): 1537-1542. DOI: 10.3760/cma.j.cn131368-20191123-01648
Abstract
ObjectiveStudy on the changes of reactive oxygen species (ROS), malondialdehyde (MDA), interleukin-6 (IL-6) and tumor necrosis factor α(TNF-α) in lung tissue and skeletal muscle cells of chronic obstructive pulmonary disease (COPD) model induced by passive smoking in rats.
MethodsAccording to the random number table method, the eight-week-old male SD rats in a SPF grade were randomly divided into COPD model group and normal control group, 10 rats in each group.Passive smoking was used in the COPD model group 8 times a day, 1 h interval, continuous passive smoking for 4 months.Pathological changes in lung tissue were observed by HE staining.Lung function were measured by small animal ventilator.The content of MDA was detected by thiobarbituric acid method.The contents of ROS, MDA, IL-6 and TNF-α were detected by enzyme linked immunosorbent assay.
ResultsBy analysis of lung histopathology and pulmonary function, the COPD model induced by passive smoking in rats for 4 month was successfully established.The contents of ROS, MDA, IL-6 and TNF-α in bronchoalveolar lavage fluid were significantly increased, there was a significant difference compared with control group (t=30.135, 16.489, 26.549, 25.346, all P<0.001). The contents of ROS, MDA, IL-6 and TNF-α in lung tissue were significantly increased, there was a significant difference compared with control group (t=25.632, 15.551, 23.422, 27.319, all P<0.001). The contents of ROS, MDA, IL-6 and TNF-α in peripheral blood serum were significantly increased, there was a significant difference compared with control group (t=24.146, 10.905, 17.327, 24.243, all P<0.001). The contents of ROS, MDA, IL-6 and TNF-α in skeletal muscle were significantly increased, there was a significant difference with control group (t=18.082, 7.573, 15.832, 21.206, all P<0.001).
ConclusionsThe contents of ROS, MDA, IL-6 and TNF-α in lung tissue and skeletal muscle are significantly increased during the development of COPD.Meanwhile, the contents of ROS, MDA, IL-6 and TNF-α in extra-pulmonary skeletal muscle are significantly increased by oxidative stress.The increase of ROS, MDA, IL-6 and TNF-α in skeletal muscle may be related to skeletal muscle atrophy.
Key words:
Rats; Pulmonary disease, chronic obstructive; Interleukin-6; Tumor necrosis factor-alpha; Oxidative stress
Contributor Information
He Jialin
Department of Respiratory Medicine, Shenzhen Longhua People′s Hospital, Shenzhen 518109, China
Hu Ruicheng
Department of Respiratory Medicine, Hunan Provincial People′s Hospital, Changsha 410016, China
Dai Aiguo
Changsha Medical College, Changsha 410000, China
