Original Article
Effects of particulate matter and cigarette-smoke extract on airway epithelium and macrophages
Yu Tao, Cui Han, Cui Ye, Liu Jie, Lyu Zhe, Sun Ying, Wang Wei, Yang Ting
Published 2020-12-20
Cite as Int J Respir, 2020, 40(24): 1841-1848. DOI: 10.3760/cma.j.cn131368-20200317-00168
Abstract
ObjectiveTo investigate the effects of particulate matter(PM) and cigarette-smoke extract (CSE) on the function of human airway epithelial cells (A549) and monocyte (THP-1)-derived macrophages.
MethodsIn vitro A549 cells and THP-1 cells were exposed to different concentrations of PM and CSE, either alone or in combination.CCK-8 kit, flow cytometry and enzyme linked immunosorbent assay were employed to measure cell proliferation, apoptosis and production of inflammatory factors in these cells, respectively.Furthermore, real-time polymerase chain reaction and Western blot were used to measure mRNA encoding macrophage polarization markers and epithelial tight junction proteins (ZO-1, occludin and claudin).
ResultsCompared with the controls, CSE (0.5%) and PM (50 mg/L) alone inhibited the proliferation of A549 cells, which was further attenuated by the presence of CSE and PM together.Co-stimulation with CSE and PM was able to enhance inhibition of cell proliferation, apoptosis, and production of inflammatory cytokines interleukin-6 and interleukin-1β in these cells.PM alone or combination of PM and CSE could reduce the expression of occludin in A549 cells.Real-time polymerase chain reaction result showed that the combination of CSE and PM further promoted CD80 mRNA expression in THP-1 cells.
ConclusionsPM can further aggravate inhibition of airway epithelial proliferation, apoptosis, inflammation and induce polarization of macrophages to M1 types.
Key words:
Particulate matter; Cigarette-smoke extract; Epithelial cells; Macrophages
Contributor Information
Yu Tao
Department of Pulmonary and Critical Care Medicine, China-Japan Friendship Hospital, National Clinical Research Center for Respiratory Diseases, Institute of Respiratory Medicine, Chinese Academy of Medical Science, Beijing 100029, China
Cui Han
Department of Physiology, Institute of Basic Medical Sciences, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100005, China
Cui Ye
Department of Immunology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China
Liu Jie
Department of Immunology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China
Lyu Zhe
Department of Immunology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China
Sun Ying
Department of Immunology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China
Wang Wei
Department of Immunology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China
Yang Ting
Department of Pulmonary and Critical Care Medicine, China-Japan Friendship Hospital, National Clinical Research Center for Respiratory Diseases, Institute of Respiratory Medicine, Chinese Academy of Medical Science, Beijing 100029, China
