廖建兰; 朱灿辉; 熊海玲; 刘腊梅; 夏美芬

doi:10.3760/cma.j.cn131368-20220923-00839

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急性呼吸窘迫综合征患儿血清lncRNA SNHG5水平与疾病严重程度及预后的关系

国际呼吸杂志, 2023,43(1) : 63-67. DOI: 10.3760/cma.j.cn131368-20220923-00839

摘要

目的

研究血清长链非编码RNA核仁小RNA宿主基因5(lncRNA SNHG5)水平在评估ARDS患儿疾病严重程度及预后中的作用。

方法

本研究为队列研究，采取非随机抽样的方法选取湖南航天医院新生儿科新生ARDS患儿98例为研究对象，根据ARDS患儿首次胸片结果及疾病严重程度分为轻度组26例、中度组39例、重度组33例；根据患儿住院治疗期间预后情况分为预后良好组75例和预后不良组23例。采用定量反转录聚合酶连锁反应法检测血清lncRNA SNHG5水平；绘制受试者工作特征曲线分析血清lncRNA SNHG5水平预测ARDS患儿预后的价值；采用多因素logistic回归分析ARDS患儿预后不良的影响因素。

结果

不同疾病严重程度ARDS患儿的lncRNA SNHG5水平比较有统计学意义(F＝42.51，P<0.001)，其中重度组ARDS患儿血清lncRNA SNHG水平(0.27±0.09)低于中度组(0.45±0.16)和轻度组(0.64±0.20)，中度组血清lncRNA SNHG5水平低于轻度组(均P<0.05)。预后不良组血清lncRNA SNHG5水平低于预后良好组[(0.29±0.09)比(0.46±0.18)，t＝4.35，P<0.001]。预后不良组胎膜早破比例低于预后良好组[8.70%(2/23)比38.67%(29/75)χ²＝7.31，P＝0.007]；预后不良组孕母妊娠期高血压比例高于预后良好组[26.09%(6/23)比1.33%(1/75)，χ²＝16.26，P<0.05]。血清lncRNA SNHG5水平最佳截断点为0.34时，预测ARDS患儿预后不良的曲线下面积为0.832(95%CI：0.751~0.913)，敏感度为76.0%，特异度为87.0%。lncRNA SNHG5增高是ARDS患儿预后不良的保护因素(95%CI：0.725~0.954，P＝0.009)。

结论

血清lncRNA SNHG5水平降低与ARDS患儿病情进展及预后不良有关，且对评估预后有价值。

引用本文: 廖建兰, 朱灿辉, 熊海玲, 等. 急性呼吸窘迫综合征患儿血清lncRNA SNHG5水平与疾病严重程度及预后的关系 [J] . 国际呼吸杂志, 2023, 43(1) : 63-67. DOI: 10.3760/cma.j.cn131368-20220923-00839.

参考文献导出: Endnote NoteExpress RefWorks NoteFirst 医学文献王

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未经授权，不得转载、摘编本刊文章，不得使用本刊的版式设计。

除非特别声明，本刊刊出的所有文章不代表中华医学会和本刊编委会的观点。

ARDS是一种与血管渗漏、肺泡充盈和缺氧相关的炎症性肺损伤^[1]。新生儿ARDS是新生儿呼吸窘迫的常见原因，其主要影响早产儿，很少影响足月儿^[2]。ARDS患儿有一定的死亡风险，这主要取决于疾病的严重程度^[3]。因此临床中寻找ARDS相关疾病进展和预后评估标准，可能对改善预后有效。研究显示ARDS患儿血清中长链非编码RNA核仁小RNA宿主基因5(long non-coding RNA small nucleolar RNA host gene 5，lncRNA SNHG5)表达下调，且参与ARDS炎症反应及细胞活力^[4]。然而关于lncRNA SNHG5与ARDS疾病严重程度及预后的关系仍未进行进一步探究。基于此，本研究旨在分析lncRNA SNHG5对ARDS患儿疾病严重程度及预后的评估作用，以期为改善患儿预后提供参考。

贡献者信息

廖建兰

湖南航天医院新生儿科，长沙　410000

朱灿辉

湖南航天医院新生儿科，长沙　410000

熊海玲

湖南航天医院新生儿科，长沙　410000

刘腊梅

湖南航天医院新生儿科，长沙　410000

夏美芬

湖南航天医院新生儿科，长沙　410000

通信作者

廖建兰

湖南航天医院新生儿科，长沙　410000

Email：1900653552@qq.com

关键词

呼吸窘迫综合征; 婴儿，新生; 基因; 预后;

作者声明

作者贡献声明

廖建兰：设计研究方案、实施研究过程、论文撰写、论文修改；朱灿辉：提出研究思路、分析试验数据、论文审核；熊海玲：实施研究过程、资料搜集整理、论文修改；刘腊梅：进行统计学分析；夏美芬：课题设计、论文撰写

利益冲突

所有作者声明无利益冲突

历史

出版日期：2023-01-25

收稿日期：2022-09-23

本文编辑

王秋红

Original Article

Relationship between serum lncRNA SNHG5 level and disease severity and prognosis in children with acute respiratorydistress syndrom

Liao Jianlan, Zhu Canhui, Xiong Hailing, Liu Lamei, Xia Meifen

Published 2023-01-25

Cite as Int J Respir, 2023, 43(1): 63-67. DOI: 10.3760/cma.j.cn131368-20220923-00839

Abstract

Objective

To study the role of serum long non-coding RNA small nucleolar RNA host gene 5 (lncRNA SNHG5) in assessing disease severity and in prognosis in children with acute respiratory distress syndrome (ARDS).

Methods

This was a cohort study.Non-random sampling method was used.Ninety-eight children with ARDS in the Newborn Department of Hunan Aerospace Hospital were selected as study subjects.According to the first chest radiograph results of children with ARDS and the severity of the disease, they were divided into mild group (26 cases), moderate group (39 cases), and severe group (33 cases); according to the prognosis during hospitalization, the children were classified into good prognosis group (75 cases) and poor prognosis group (23 cases). qRT-PCR was used to measure the serum lncRNA SNHG5 level; ROC curve was drawn to analyze the value of serum lncRNA SNHG5 level in predicting the prognosis of children with ARDS; multivariate Logistic regression was used to analyze the influencing factors of poor prognosis in children with ARDS.

Results

The level of lncRNA SNHG in children with ARDS of different disease severity was statistically significant (F=42.51, P<0.001). The level of serum lncRNA SNHG in severe group (0.27±0.09) was lower than that in moderate group (0.45±0.16) and mild group (0.64±0.20), with the level in moderate group lower than that in mild group (P<0.05). The level of serum lncRNA SNHG5 in poor prognosis group (0.29±0.09) was lower than that in good prognosis group (0.46±0.18)(t=4.35, P<0.001). The proportion of premature rupture of membranes in poor prognosis group was lower than that in good prognosis group, with 8.70% (2/23) vs 38.67% (29/75), (χ²=7.31, P=0.007). The proportion of pregnancy induced hypertension in poor prognosis group was 26.09% (6/23), higher than that in good prognosis group 1.33% (1/75), (χ²=16.26, P<0.05). When the optimal cut-off point of serum lncRNA SNHG5 level was 0.34, the area under the curve to predict the poor prognosis of children with ARDS was 0.832 (95% CI: 0.751-0.913), the sensitivity was 76.0%, and the specificity was 87.0%.Increased lncRNA SNHG5 is a protective factor for poor prognosis in children with ARDS (95% CI: 0.725-0.954, P=0.009).

Conclusions

Decreased level of serum lncRNA SNHG5 can lead to disease progression and poor prognosis of children with ARDS, and it has a high value for prognosis evaluation.

Key words:

Respiratory distress syndrome; Infant, new-born; Genes; Prognosis

Contributor Information

Liao Jianlan