Original Article
Effect of VZV on the cellular prion protein glycosylation of Schwann cells and the regulation of methylcobalamin
Xu Gang, Zhou Chaosheng, Zhang Yu, Tang Weizhen, Cheng Chao, Xu Jie, Xu Gang, Wang Xiaomei
Published 2021-06-30
Cite as Chinese J Exp Clin Virol, 2021, 35(3): 266-271. DOI: 10.3760/cma.j.cn112866-20200904-00236
Abstract
ObjectiveTo explore the effects of varicella-zoster virus (VZV) on the glycosylation characteristics of cellular prion protein (PrPC) in human Schwann cells (hSC) and the regulation of methylcobalamin (MeB12).
MethodsThe hSC were inoculated with VZV at 1.0 multiplicity of infection for 48 hours, then 250 μg/ml of MeB 12 were added and cultured for 48 hours. PrPC from the supernatant and sediment were coated with anti-PrPC antibody (3F4) respectively and subjected to screening for glycans by sandwich lectin-ELISA. Meanwhile, the superoxide dismutase (SOD) and malondialdehyde (MDA) from the supernatant were detected by diagnostic reagent kit.
ResultsThe ratio of PrPC glycans in the supernatant to sediment of VZV-infected cells was found to be significantly different compared with those in the VZV-non-infected cells. The overall glycans ratios of the supernatant to the sediment was 1∶2.6 in the uninfected cells, while the ratio was 1∶1.5 in the VZV-infected cells (F=24.18, P<0.001, LSD-t=8.27,P<0.001), suggesting that stability of PrPC decreased after VZV infection, and correspondingly the activity of SOD (4.43±2.05 U/mg) was significantly reduced in the VZV-infected hSCs compared with those(14.23±1.27 U/mg) in the uninfected cells (F=18.19, P=0.001, LSD-t=6.54, P<0.001), the level of MDA (11.17±1.89 nmol/mg) was significantly elevated in the VZV-infected hSCs compared with those (3.73±0.35nmol/mg) in the uninfected cells (F=30.70,P<0.001, LSD-t=8.25,P<0.001). When the VZV-infected cells were added with 250 μg/ml MeB12, glycans in the sediment of infected cells significantly increased compared with those in the VZV-infected cells without MeB12, the overall glycans ratio of the supernatant to the sediment was 1∶2.4, suggesting that MeB12 improved the stability of PrPC. Moreover, SOD activity (11.07±2.07 U/mg) was significantly increased (LSD-t=4.42, P=0.002), MDA level (5.23±0.96 nmol/mg) was significantly decreased (LSD-t=6.58, p<0.001) in the VZV-infected cells added with MeB12 compared with those in the VZV-infected cells without MeB12.
ConclusionsThe glycosylation characteristics of PrPC in hSC could be changed by VZV, while MeB12 could regulate the glycosylation characteristics to improve the stability of PrPC, thereby increase the antioxidant capacity of hSC.
Key words:
Varicella-zoster virus; Human Schwann cells; Cellular prion protein; Glycosylation; Methylcobalamin
Contributor Information
Xu Gang
Zoster-Associated Pain Center, Affiliated Tenth People′s Hospital of Tongji University, Shanghai Tenth People′s Hospital, Shanghai 200072, China
Zhou Chaosheng
Zoster-Associated Pain Center, Affiliated Tenth People′s Hospital of Tongji University, Shanghai Tenth People′s Hospital, Shanghai 200072, China
Zhang Yu
Zoster-Associated Pain Center, Affiliated Tenth People′s Hospital of Tongji University, Shanghai Tenth People′s Hospital, Shanghai 200072, China
Tang Weizhen
Zoster-Associated Pain Center, Affiliated Tenth People′s Hospital of Tongji University, Shanghai Tenth People′s Hospital, Shanghai 200072, China
Cheng Chao
Zoster-Associated Pain Center, Affiliated Tenth People′s Hospital of Tongji University, Shanghai Tenth People′s Hospital, Shanghai 200072, China
Xu Jie
Zoster-Associated Pain Center, Affiliated Tenth People′s Hospital of Tongji University, Shanghai Tenth People′s Hospital, Shanghai 200072, China
Xu Gang
Zoster-Associated Pain Center, Affiliated Tenth People′s Hospital of Tongji University, Shanghai Tenth People′s Hospital, Shanghai 200072, China
Wang Xiaomei
Zoster-Associated Pain Center, Affiliated Tenth People′s Hospital of Tongji University, Shanghai Tenth People′s Hospital, Shanghai 200072, China
