Infection and Inflammation
Analysis of characteristics and related risk factors of systemic inflammatory response syndrome caused by wasp stings
Xiao Min, Yao Wei, Sun Yuhui, Chen Ping, Chen Dongli, Sun Yuwen, Wang Chuanlin, Wang Ke
Published 2021-02-15
Cite as Chin J Trauma, 2021, 37(2): 146-151. DOI: 10.3760/cma.j.cn501098-20200709-00494
Abstract
ObjectiveTo analyze the risk factors of systemic inflammatory response syndrome (SIRS) caused by wasp stings.
MethodsA prospective case-control study was conducted to analyze the clinical data of 225 patients with wasp stings admitted to Taihe hospital of Shiyan City and Ankang Central Hospital from January 2014 to December 2018, including 131 males and 94 females, with the age of 49 (41, 60) years. The patients were divided into SIRS group (n=62) and non-SIRS group (n=163) according to the SIRS diagnostic criteria. The data were collected including gender, age, sting in the head, sting in waist and back, sting in the abdomen, sting in the limbs, number of stings, admission time, hospitalization day, and mortality. The plasma levels of interleukin(IL)-6 and IL-8 in peripheral blood of the patients were analyzed by ELISA method. The whole genome DNA was extracted from white blood cells, and the IL-6: -174G/C, -572G/C, -597G/A, -634C/G and IL-8: -251A/T, -738T/A, -845T/C, + 396T/G were selected for research. The PCR method was used to perform bidirectional sequencing and comparison after amplification, and record the genotyping and frequency. The risk factors of SIRS caused by wasp stings were investigated by univariate analysis and multivariate Logistics regression analysis.
Results(1) The two groups showed significant differences in sting in the limbs, sting in waist and back, sting in the abdomen, number of stings, hospitalization day and mortality (P<0.01), while there were no significant differences in gender, age, sting in the head and admission time (P>0.05). (2) ELISA test showed the plasma levels of IL-6 and IL-8 in SIRS group were higher than those in non-SIRS group (P<0.01). (3) Three genotypes of CC, GC and GG were observed in IL-6-572G/C. There were significant differences in the frequencies of genotypes and alleles between SIRS group and non-SIRS group (P<0.01). Three genotypes of AA, AT and TT were observed in IL-8 -251A/T. There were significant differences in the frequencies of genotypes and alleles between SIRS group and non-SIRS group (P<0.01). (4)Univariate analysis showed sting in waist and back, sting in the abdomen, sting in the limbs, number of stings, IL-6-572G allele, IL-8-251T allele were related to SIRS caused by wasp stings (P<0.01). (5)Multivariate Logistics regression analysis showed limb stings (OR=2.15), number of stings ≥10 (OR=11.10), IL-6-572G allele (OR=3.91) and IL-8-251T allele (OR=3.97) were significantly related to SIRS caused by wasp stings (P<0.05 or 0.01).
ConclusionsThe plasma levels of IL-6 and IL-8 are increased in patients with SIRS after wasp stings. The limbs stings, number of stings ≥10, IL-6 -572G and IL-8 -251T are all independent risk factors for SIRS caused by wasp stings.
Key words:
Wasps; Interleukins; Systemic inflammatory response syndrome; Risk factors
Contributor Information
Xiao Min
Department of Critical Care Medicine, Tianyou Hospital Affiliated to Wuhan University of Science &
Technology, Wuhan 430064, China
Yao Wei
Departmen of Nephrology, Jingzhou Central Hospital, Jingzhou 434020, China
Sun Yuhui
Departmen of Pediatrics, Taihe Hospital of Shiyan City, Shiyan 442000, China
Chen Ping
Department of Critical Care Medicine, Tianyou Hospital Affiliated to Wuhan University of Science &
Technology, Wuhan 430064, China
Chen Dongli
Department of Emergency, Ankang Central Hospital, Ankang 725000, China
Sun Yuwen
Department of Emergency, Taihe Hospital of Shiyan City, Shiyan 442000, China
Wang Chuanlin
Department of Emergency, Peking University People's Hospital, Beijing 100044, China
Wang Ke
Department of Critical Care Medicine, Tianyou Hospital Affiliated to Wuhan University of Science &
Technology, Wuhan 430064, China
