To study the effects of cigarette smoke on fertility of male mice.

The C57/BL6J male mice were treated with cigarette smoke twice a day in a 30 cm×30 cm×30 cm air-tight cabinet, lasting for 6 weeks. The cotinine content in smoking mouse serum was detected by ELISA, along with the monitor of the oxygen content of smoking mouse, the sperm mobility and progressive motility, as well as the average birth number and the survival rate of the offspring of the smoking mice.

The cotinine content in smoking mouse serum was 16.07±3.52 ng/ml and was equivalent to the cotinine content of human who smoked 20 cigarettes a day. The pO2 was down when it's smoking, but it would recover after cigarette smoking. The sperm mobility and progressive motility of the smoking mice were both higher than those of no smoking mice. The average birth number and the survival rate of the offspring of the smoking mice were statistically significantly lower than those of no smoking mice. The mouse smoking model was considered to be credible.

Our mouse smoking model was similar to the people who smoked 20 cigarettes a day, so this model could make contributions to the investigation of the smoking effect on reproductive system in smoking mice.

cigarette smoke; mouse passive smoking model; sperm; fertility