Clinical Genetics
Association of genomic instability of CDH1 gene with clinicopathological characteristics of gastric cancer
Du Jitao, Wan Xiangbin, Zhang Huiliang, Cao Jian, Zhao Wen, Li Zhi
Published 2022-11-10
Cite as Chin J Med Genet, 2022, 39(11): 1279-1282. DOI: 10.3760/cma.j.cn511374-20210304-00186
Abstract
ObjectiveTo assess the association of genomic instability of epithelial cadherin 1 (CDH1) gene and clinicopathological characteristics of gastric cancer.
MethodsIn total 120 paraffin-embedded gastric cancer tissue specimen were prepared, and genomic DNA was extracted. The genomic instability of the CDH1 gene was analyzed by immunohistochemistry and silver staining PCR-single-strand conformation polymorphism.
ResultsThe number of information individuals (heterozygotes) was 98 for the D16S752 locus. The detection rates for microsatellite instability (MSI) and loss of heterozygosity (LOH) at the D16S752 locus and the positive rate of CDH1 protein were 19.39%, 16.33% and 51.02%, respectively. The detection rate of MSI in TNM stages Ⅰ or Ⅱ was significantly higher than that in stages Ⅲ or Ⅳ (P<0.05) while the detection rate of LOH was significantly lower than that in stages Ⅲ or Ⅳ (P<0.05). The positive rate of CDH1 protein in TNM stages Ⅲ or Ⅳ was significantly lower than that in stages Ⅰ or Ⅱ (P<0.05). The detection rate of MSI of cases with lymph node metastasis was significantly lower than that of without lymph node metastasis (P<0.05) while the detection rate of LOH was significantly higher than that without lymph node metastasis (P<0.05). The positive rate of CDH1 protein in patients with lymph node metastasis was significantly lower than that in patients without lymph node metastasis (P<0.05). The positive rate of CDH1 protein in MSI-positive group was significantly higher than that in MSI-negative group (P<0.05), and the positive rate of CDH1 protein in the LOH-positive group was significantly lower than that the LOH-negative group (P<0.05).
ConclusionThe genomic instability of the CDH1 gene is associated with the progression of gastric cancer. MSI at the D16S752 locus may be used as a molecular marker for early gastric cancer, while LOH at this locus mostly occurs in advanced gastric cancer and can be regarded as an effective indicators for malignancy evaluation and prognosis.
Key words:
Gastric cancer; Epithelial cadherin; Microsatellite instability; Loss of heterozygosity; Pathological characteristics
Contributor Information
Du Jitao
Department of General Surgery, the Cancer Hospital Affiliated to Zhengzhou University, Zhengzhou, Henan 450000, China
Wan Xiangbin
Department of General Surgery, the Cancer Hospital Affiliated to Zhengzhou University, Zhengzhou, Henan 450000, China
Zhang Huiliang
Department of General Surgery, the Cancer Hospital Affiliated to Zhengzhou University, Zhengzhou, Henan 450000, China
Cao Jian
Department of General Surgery, the Cancer Hospital Affiliated to Zhengzhou University, Zhengzhou, Henan 450000, China
Zhao Wen
Department of General Surgery, the Cancer Hospital Affiliated to Zhengzhou University, Zhengzhou, Henan 450000, China
Li Zhi
Department of General Surgery, the Cancer Hospital Affiliated to Zhengzhou University, Zhengzhou, Henan 450000, China
