Review
Advances in strategies for regulating extracellular vesicle formation and biological function
Wang Zehua, Mao Xingjia, Guo Zijian, Feng Tengda, Tian Zui, Yin Zhiwen, Ge Zigang, Fu Panfeng, Xiang Chuan
Published 2023-04-08
Cite as Chin J Exp Surg, 2023, 40(4): 789-797. DOI: 10.3760/cma.j.cn421213-20220603-00418
Abstract
Extracellular vesicles (EVs) are subcellular components produced by paracrine secretion, which can deliver proteins, nucleic acids, lipids and other bioactive molecules to target cells to mediate intercellular signal exchange, regulate the biological behavior of target cells and cause corresponding functional and phenotypic changes. It has great potential value in biomedical field. However, the natural EVs secretion of non-tumor cells is very low, which makes it difficult to meet the clinical therapeutic dose requirements. Moreover, with the aging of donor cells, the EVs secretion decreases sharply, and the biological function of its contents also changes uneasily. Therefore, it is of great significance for the clinical application and promotion of EVs to increase EVs secretion and accurately regulate its biological function. In this paper, the structure and formation process of extracellular vesicles are briefly summarized, and the methods and applications that affect the biological behavior of donor cells, promote the generation of EVs and enhance the required biological functions are described in detail, hoping to provide new ideas for improving the extraction efficiency of EVs and the application and promotion of EVs therapy in clinical practice.
Key words:
Extracellular vesicles; Exosomes; Mesenchymal stem cells; Therapy
Contributor Information
Wang Zehua
Department of Bone, the Second Hospital of Shanxi Medical University, Taiyuan 030001, China
Mao Xingjia
School of Basic Medical Science, Zhejiang University, Hangzhou 310058, China
Guo Zijian
Department of Bone, the Second Hospital of Shanxi Medical University, Taiyuan 030001, China
Feng Tengda
Department of Bone, the Second Hospital of Shanxi Medical University, Taiyuan 030001, China
Tian Zui
Department of Bone, the Second Hospital of Shanxi Medical University, Taiyuan 030001, China
Yin Zhiwen
Department of Bone, the Second Hospital of Shanxi Medical University, Taiyuan 030001, China
Ge Zigang
Colleage of Engineering, Peking University, Beijing 100871, China
Fu Panfeng
Department of Biochemistry and Molecular Biology, Ningbo University, Ningbo 315211, China
Xiang Chuan
Department of Bone, the Second Hospital of Shanxi Medical University, Taiyuan 030001, China
