Experimental Study
Preparation of ellagic acid nano supplementation and their anti-tumor effect in breast cancer MCF-7 cell lines
Lin Xiao, Zhang Qingxia, Wang Jinglong, Yang Fuxia, Xu Lili
Published 2023-08-08
Cite as Chin J Exp Surg, 2023, 40(8): 1554-1557. DOI: 10.3760/cma.j.cn421213-20230107-01006
Abstract
ObjectiveTo prepare an ellagic acid nano supplementation and observe its effect on the behavioral characteristics of MCF-7 cell lines, so as to analyze whether its anti-tumor effect has been improved.
MethodsChitosan nano microcapsules were prepared by layered self-assembly method, and the difference between antioxidant activity and free ellagic acid in vitro was observed. At the same time, MCF-7 cells were cultured in vitro, including MCF-7 group, free ellagic acid group and nano microcapsule group (n=3). MCF-7 group was cultured normally, free ellagic acid group and nano capsule group were co-cultured with free ellagic acid and ellagic acid nano microcapsule for 48 h, respectively. Cell counting kit-8 (CCK-8) and flow cytometry experiments were used to detect cell appreciation and apoptosis viability. The counting data were expressed by rate and constituent ratio. The comparison between the two groups was performed by Chi-squared test.
ResultsThe antioxidant test results showed that the absorbance (A)734 of ellagic acid nano supplementation was 0.652±0.134, which was significantly lower than that of 0.855±0.121 of free ellagic acid (P<0.05). The results of in vitro cytology experiments showed that the A450 values of MCF-7 group, free ellagic acid group and nano supplementation group were 0.854±0.102, 0.595±0.077 and 0.452±0.046 respectively, with the differences between the groups being significant (F=20.265, P<0.01), and those in the nano supplementation group were higher than in the free ellagic acid group (F=12.258, P<0.05), and those in the free ellagic acid group was higher than those in the MCF-7 group (F=31.024, P<0.05). The apoptosis rates in MCF-7 group, free ellagic acid group and nano supplementation group were (6.25±0.87)%, (15.63±2.22)%, and (21.52±2.54%)% respectively, with the differences between the groups being significant (F=43.985, P<0.01), those in the nano supplementation group was lower than in the free ellagic acid group (F=26.071, P<0.05), and those in the free ellagic acid group was lower than in the MCF-7 group (F=14.356, P<0.01).
ConclusionThe ellagic acid nano microcapsule was successfully prepared, and it was confirmed that its antioxidant activity and breast cancer activity in vitro were better than those of free ellagic acid.
Key words:
Nano supplementation; Ellagic acid; Breast cancer; Chitosan
Contributor Information
Lin Xiao
Department of Pharmacy, Zaozhuang Municipal Hospital, Zaozhuang 277100, China
Zhang Qingxia
Department of Pharmacy, Zaozhuang Traditional Chinese Medicine Hospital, Zaozhuang 277100, China
Wang Jinglong
School of Food Science and Pharmaceutical Engineering, Zaozhuang University, Zaozhuang 277100, China
Yang Fuxia
Department of Pharmacy, Zaozhuang Municipal Hospital, Zaozhuang 277100, China
Xu Lili
Department of Pharmacy, Zaozhuang Traditional Chinese Medicine Hospital, Zaozhuang 277100, China
