Objective To understand the effect of EGF,TGF-β1,VEGF on the anastomotic leak age after colorectal cancer surgery by the investigation of EGF 61*G/A,TGF-β1-509*T/C,VEGF 936 *T/C gene functional polymorphism.Methods PCR-RFLP was employed to study the EGF 61*G*A, TGF-β1-509*T/C,VEGF 936*T/C genotypes in colorectal cancer patients with or without anastomotic leakage after surgery.Results The frequency of TGF-β1-509*T/T genotype in colorectal cancer pa tients with anastomotic leakage(0.0)was significantly lower than that in the patients without anastomotic leakage(13.2％)(P＜0.05).The frequency of VEGF 936*C/C genotype and C allele in colorectal cancer patients with anastomotic leakage(63.9％and 80.6％respectively)was significantly lower than in the patients without anastomotic leakage(82.6％and 90.5％respectively)(genotype χ2=5.753,P＜ 0.05；allele χ2=5.275,P＜0.05).Although the EGF 61*G/G genotype in colorectal cancer patients with anastomotic leakage(25.0％)was less frequently found than in the patients without anastomotic leakage(35.5％),there was no statistically significant difference(χ2=1.391,P＞0.05).Conclusion TGF-β1-509*T/T genotype,VEGF 936*C/C genotype and C allele decrease the risk of anastomotic leakage after surgery for colorectal cancer.