Objective To establish hierarchical full-responsibility nursing system in the laminar air flow units for various stages of hematopoietic stem cell transplantation,in order to guarantee that patients receive safe,professional and seamless quality nursing service.Methods Organizational structure and responsibility as well as staff training and assessment were established.Nursing methods in various stages of transplantation were developed and carried out.Meanwhile,primary nursing and hierarchical system were combined together to provide patients with the comprehensive and brand-new nursing mode.Clinical data of 40 cases with HSCT using the mode,and 37 cases without using the mode were both gathered,and complications as well as patients' satisfaction were compared between two groups.Results After the establishment of the hierarchical full-responsibility nursing system,the incidence rates of complications including temperature rise,oral ulcer,perianal infection,hemorrhagic cystitis,central venous catheter-related infection and GVHD of the 40 cases were respectively 75.00％,52.50％,5.00％,7.50％,0.00％,42.50％,while those of the 37 cases without the system were 83.78％,78.38％,24.32％,29.73％,10.81％,45.95％.The differences were statistically significant (x2 =4.299,4.299,5.862,6.385,7.469,3.862,respectively; P ＜ 0.05).The hierarchical full-responsibility nursing system improved nurses' theory and operation achievements (90.25 ± 5.25 vs 71.20 ±5.15,and 92.55 ±5.33 vs 80.24 ±4.20),and the differences were statistically significant (t =-45.876,-22.437,respectively;P ＜ 0.01).Both patients' and doctors' satisfaction with nurses were also obviously improved (97.29％ vs 83.78％ and 95.00％ vs 80.00％),and the differences were statistically significant (x2 =3.944 6,4.114 3,respectively; P ＜ 0.05).Conclusions After the hierarchical full-responsibility nursing system is established,nurses' service consciousness,special knowledge and operation level of transplantation have been so improved that patients can receive standardized and scientific nursing during transplantation,the occurrence of complications can be deduced and the hospitalization period can be shortened.