Explore the clinical application values of Golgi Protein 73 (GP73), AFP variants (AFP-L3) , Alpha fetoprotein (AFP)and α-l-Fucosidase (AFU) detection in the diagnosis of hepatocellular carcinoma (HCC).

From January of 2013 to June of 2014, 84 case of HCC Patients(HCC group) who presented at interventional department; 64 case of cirrhotic patients(liver cirrhosis group), 86 case of chronic hepatitis patients(chronic hepatitis group) and 120 healthy people (normal control group) were selected from Shanghai Tongren Hospital. GP73 was detected by the enzyme-linked immunosorbent assay (ELISA), AFP-L3 was isolated with ACSC, AFP and AFP-L3 were detected with ECLIA and calculated the percentage content of AFP-L3 (AFP-L3%), AFU was detected with enzyme kinetic method. Adopted the SPSS 19.0 statistical software for data analysis. The rank sum test was used in the multi group comparison; the chi square test was used in the comparison group.

Serum levels of GP73, AFP-L3, AFP and AFU in HCC group were 202.1 μg/L, 9.5%, 68.3 μg/L, 33.2 μg/L respectively. Their difference from those of the normal control group(69.0 μg/L, 2.5%, 4.5 μg/L, 24.2 μg/L) was of statistical significance (U was 1126.59, 204.67, 1247.68, 564.08, respectively, all P<0.05). GP73 and AFP in the hepatic carcinoma group was significantly higher than that in the cirrhosis group(151.1 μg/L、18.5 μg/L) (U was 463.47, 368.56, respectively, P<0.05). GP73, AFP-L3 and AFP in the hepatic carcinoma group was significantly higher than that in the chronic hepatitis group(93.6 μg/L, 3.5%, 5.7 μg/L) (U was 1 116.52, 213.42, 1 166.52, respectively, all P<0.05). The content of AFU in HCC group (33.2 μg/L), normal control group and chronic hepatitis group were compared (24.2 μg/L, 24.1 μg/L), respectively , no significance was found(U was 564.08, 487.24, P>0.05) Sensitivity of GP73 and AFP in individual inspection was 95.24%, significantly higher than that of AFU, AFP-L3. Specificity of AFP-L3 was 94.81% respectively, with an accuracy of 85.88% respectively. Specificity and accuracy of the allied detection of GP73, AFP-L3, AFP and AFU for HCC diagnosis were 98.52% and 84.75% respectively.

The allied combination of serum GP73, AFP-L3, AFP and AFU makes up for the insufficient clinical applications of individual serum markers. It is of great clinical significance to improve the diagnosis of HCC.(Chin J Lab Med, 2016, 39: 262-266)

Golgi apparatus; alpha-Fetoproteins; alpha-L-fucosidase; Liver neoplasms; Diagnosis