Multiple sclerosis (MS) is an autoimmune central nervous system disease, which is caused by loss of autoimmune tolerance. Although the mechanism of impaired self-tolerance has not been fully elucidated, related studies have shown that regulatory T cells (Treg) are closely related to the pathogenesis of MS. Treg cells can inhibit inflammation and autoimmune response by inhibiting the proliferation and activation of effector T cell subsets in vivo and maintaining autoimmune tolerance. The restoration and enhancement of Treg cell function has potential value in improving MS disease. Therefore, the current research progress of regulatory T cells in MS is reviewed.