Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is the most popular method for the study of protein-chromatin interaction . However, it is difficult to implement on a low start-ing number of cells or single-cell application. The chromatin cleavage under target sequencing technology emerged in recent years has shown advantages such as simple operation, low background, high reproducibi-lity, and low starting cell numbers. It can replace the traditional ChIP-seq and improve single-cell Chip-seq method. This article briefly reviews the development and application situation of this type of chromatin cleavage under target sequencing technology.