Original Article
The effect of IGF2 on early mouse embryo development in vitro
Yu Qingmei, Zhang Li, Ding Xiangyun, Li Shanshan, Gao Gang
Published 2021-06-15
Cite as Int J Genet, 2021, 44(3): 141-145. DOI: 10.3760/cma.j.cn231536-20200730-00069
Abstract
ObjectiveTo observe the effect of IGF2 on mouse early embryos in vitro development.
Methods① In vitro culture: Take fertilized eggs from mice in the control group and IMP2 knockout group, culture them continuously in M16 medium with or without IGF2, and then observe and calculate number and rate of blastocyst development in each group; ② Embryo transfer: Transferring the four groups early embryos back to surrogate female mice, and observing the birth rate and pregnancy rate of the four surrogate female mouse groups.
ResultsIn vitro culture, the blastocyst formation rate was 29.4% without IGF2 and 65.8% with IGF2 (P=0.002). The IMP2 knockout groups rarely developed to blastocysts(P=0.060). In embryo transfer experiment, the surrogate mothers that received IGF2 embryos gave birth to more pups per female on average, and the pregnancy rate was significantly higher than that of control group(P<0.001).
ConclusionIGF2 significantly increased blastocyst formation rate and embryonic development ability in control group, but had no effect on IMP2 gene knockout mice.
Key words:
IGF2 protein; Mouse; Early embryo; In vitro development
Contributor Information
Yu Qingmei
Department of Anatomy, Shandong Medical College, Linyi 276000, China
Zhang Li
Genetic Laboratory, Linyi City Maternal and Child Health Hospital, Linyi 276000, China
Ding Xiangyun
Department of Anatomy, Shandong Medical College, Linyi 276000, China
Li Shanshan
Department of Anatomy, Shandong Medical College, Linyi 276000, China
Gao Gang
Genetic Laboratory, Linyi City Maternal and Child Health Hospital, Linyi 276000, China
