Original Article
Studies on drug and disinfectant resistance related genes of Yersinia pestis in China
Dai Ruixia, He Jian, Yang Xiaoyan, Xin Youquan, Li Sheng, Jin Juan, Zhang Qi, Bai Jixiang, Wei Baiqing
Published 2021-01-20
Cite as Chin J Endemiol, 2021, 40(1): 27-31. DOI: 10.3760/cma.j.cn231583-20191024-00295
Abstract
ObjectiveTo understand whether there are drug resistant and disinfectant resistant Yersinia pestis strains in China, and to provide accurate information for clinical treatment of plague.
MethodsA total of 2 753 Yersinia pestis strains isolated from 10 natural plague foci in China from 1943 to 2016 were collected. According to National Center for Biotechnology Information (NCBI) released sequences of aminoglycoside streptomycin resistant genes strA, strB, β-lactam antibiotics resistant genes TEM, SHV and CTX-M, sulfamilamide resistant genes sul1, sul2 and sul3, and disinfectant resistant gene qacE△1-sul1, a pair of primers of each gene was designed for above-mentioned genes. Genomic DNA of 2 753 strains of Yersinia pestis was extracted, and the 9 target genes of all DNA samples were amplified by PCR.
ResultsNegative and positive controls of PCR detection were established. No corresponding target bands of aminoglycoside streptomycin resistant genes strA, strB, β-lactam antibiotics resistant genes TEM, SHV and CTX-M, sulfamilamide resistant genes sul1, sul2 and sul3, and disinfectant resistant gene qacE△1-sul1 were found in the DNA samples of 2 753 strains of Yersinia pestis.
ConclusionThe above-mentioned genes of drug resistance and disinfectant resistance have not been detected in Yersinia pestis of China, but the monitoring of drug resistance of Yersinia pestis still needs to be carried out continuously.
Key words:
Yersina pestis; Drug tolerance; Disinfectant
Contributor Information
Dai Ruixia
Yersina Pestis Laboratory, Qinghai Institute for Endemic Disease Prevention and Control, Xining 810021, China
He Jian
Yersina Pestis Laboratory, Qinghai Institute for Endemic Disease Prevention and Control, Xining 810021, China
Yang Xiaoyan
Yersina Pestis Laboratory, Qinghai Institute for Endemic Disease Prevention and Control, Xining 810021, China
Xin Youquan
Yersina Pestis Laboratory, Qinghai Institute for Endemic Disease Prevention and Control, Xining 810021, China
Li Sheng
Yersina Pestis Laboratory, Qinghai Institute for Endemic Disease Prevention and Control, Xining 810021, China
Jin Juan
Yersina Pestis Laboratory, Qinghai Institute for Endemic Disease Prevention and Control, Xining 810021, China
Zhang Qi
Yersina Pestis Laboratory, Qinghai Institute for Endemic Disease Prevention and Control, Xining 810021, China
Bai Jixiang
Yersina Pestis Laboratory, Qinghai Institute for Endemic Disease Prevention and Control, Xining 810021, China
Wei Baiqing
Plague Prevention and Control Department, Qinghai Institute for Endemic Diseases Prevention and Control, Xining 810021, China
