Basic Research
Experimental study on diabetic cardiomyopathy rats treated with NMFGF1 loaded PEG-modified nano-liposomes combined with ultrasound-targeted microbubble destruction technique
Ming Zhang, Yanhua Yu, Jingling Wang, Yuanna Chen, Jinlong Xu, Mengjia Chen, Lu Yu, Shufang Yu, Weicheng Ma
Published 2019-07-25
Cite as Chin J Endocrinol Metab, 2019, 35(7): 599-605. DOI: 10.3760/cma.j.issn.1000-6699.2019.07.011
Abstract
ObjectiveTo investigate the therapeutic effect and mechanism of non-mitogenic acid fibroblast growth factor 1(NMFGF1) on diabetic cardiomyopathy (DCM) by using PEG-modified nano-liposomes combined with ultrasound-targeted microbubble destruction technique (UTMD).
MethodsThe NMFGF1 loaded PEG-modified nano-liposomes were prepared by a water-in-water emulsion method and their quality inspections were also investigated. Type 1 diabetes animal model was induced by intraperitoneal injection of streptozotocin (70 mg/kg) in male SD rats. The diabetic rats were raised twelve weeks after the diabetes model was established and DCM rats were selected by ultrasonic heart function examination. After two weeks of intervention, all rats were kept for another two weeks and then underwent transthoracic echocardiography examination. The rats were sacrificed and myocardial tissue was obtained to quantify myocardial collagen fraction (CVF) and cardiac myocyte apoptotic index by Sirius red staining and TUNEL staining.
ResultsNMFGF1-loaded PEG-nano-liposomes showed a good morphology and 90.3%±1.4% NMFGF1 encapsulation efficiency. Compared with DCM group, NMFGF1group, and NMFGF1-PEG-nano-liposomes group, NMFGF1-loaded PEG-nano-liposome plus UTMD group showed increased left ventricular end diastolic diameter (LVIDd) [(7.36±0.42) vs (5.75±0.24), (6.64±0.27), (6.72±0.24)mm, all P<0.05]and left ventricular fraction shortening (LVFS) [(50±3) vs (33±2), (44±5), (43±3)mm, all P<0.05], and decreased left ventricular posterior wall thickness (LVPW) [(1.65±0.07) vs (1.89±0.08), (1.73±0.11), (1.73±0.07)mm, all P<0.05], with decreased CVF and apoptotic index(all P<0.05).
ConclusionPEG-nano-liposomes combining with UTMD technique has a greater translational potential in the delivery of NMFGF1 for the treatment of DCM by attenuating oxidative stress-induced injury and may provide a promising strategy for treating diabetes cardiomyopathy.
Key words:
Diabetic cardiomyopathies; Oxidative stress; Non-mitogenic acid fibroblast growth factor 1; Ultrasound-targeted microbubble destruction technique; PEG -nano-liposomes
Contributor Information
Ming Zhang
Pharmaceutical Department, Ningbo Yinzhou Second Hospital, Ningbo 315100, China
Yanhua Yu
Jingling Wang
Yuanna Chen
Jinlong Xu
Mengjia Chen
Lu Yu
Shufang Yu
Weicheng Ma
