Diagnosis of Aldosteronism
Application of CYP11B2 immunohistochemistry in the histopathological diagnosis of primary aldosteronism
Yi Yang, Ming Xiao, Ying Song, Jiayu Li, Qingfeng Cheng, Shumin Yang, Jinbo Hu, Zhipeng Du, Qifu LI
Published 2019-11-25
Cite as Chin J Endocrinol Metab, 2019, 35(11): 939-944. DOI: 10.3760/cma.j.issn.1000-6699.2019.11.004
Abstract
ObjectiveTo evaluate the value of CYP11B2 immunohistochemistry in the histopathological diagnosis of primary aldosteronism(PA) among Chinese population.
MethodsNinety-six cases of unilateral PA patients were retrospectively enrolled, and with 15 cases of non-hyperfunctioning adenoma, and 15 cases of cortisol producing adenoma as control. CYP11B2 immunohistochemistry was performed in the tumor sections of these patients and was analysed by semi-quantified method. The positive rate of CYP11B2 in tumor specimen of all cases enrolled was calculated and PA patients were subtypely classified based on their histopathological features and CYP11B2 expressions.
ResultsThe immune-positive rate of CYP11B2 in the PA group was 91.7% (88/96), with the control group being 6.7% (2/30, P<0.001). Subgroup analysis revealed that the immune-positive rate of CYP11B2 in the aldosterone producing adenoma group and unilateral hyperplasia group were 91.8% (78/85) and 90.9% (10/11), respectively. Based upon histopathological feature and CYP11B2 immunohistochemistry, PA patients were further subtypely classified as aldosterone producing adenoma (85/96), unilateral diffuse adrenocortical hyperplasia (7/96), unilateral mono-adrenocortical micronodules (1/96), unilateral multiple adrenocortical micronodules (1/96), and unilateral multiple aldosterone-producing cell clusters (multiple APCCs, 2/96).
ConclusionCYP11B2 immunohistochemistry may serve as an useful tool for the histopathological diagnosis of PA.
Key words:
Primary aldosteronism; Immunohistochemistry; Pathological diagnosis
Contributor Information
Yi Yang
Department of Endocrinology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
Ming Xiao
Department of Pathology, College of Basic Medicine, Chongqing Medical University, Chongqing 400016, China
Ying Song
Department of Endocrinology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
Jiayu Li
Chongqing Colleges and Universities Key Laboratory of Major Metabolic Disease and Translational Medicine
Qingfeng Cheng
Department of Endocrinology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
Shumin Yang
Department of Endocrinology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
Jinbo Hu
Department of Endocrinology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
Zhipeng Du
Department of Endocrinology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
Qifu LI
Department of Endocrinology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
