Human placental trophoblast cell (TB) can be classified into villous cytotrophoblast cell (VCT) , villous syncytiotrophoblast cell (VST) , and extrvillous trophoblast cell (EVT) . Varieties of TB are differential in morphology, and play disparate roles in the physiological or pathological processes. Besides inevitable maternal cell contamination, it is necessary to highlight the purity of TB in researches, in which also exists the contradiction of vitality and purity problems. Density gradient centrifugation, immunomagnetic separation and flow cytometry (FCM) have been used widely in the sorting of cells. There are advantages and disadvantages for all of these methods, however, immunomagnetic separation combined with FCM appears to be more effective among all the sorting tactics.