To verify and evaluate the feasibility of the solvent/detergent (S/D) method combined with nanomembrane filtration method in the preparation of human plasma antithrombin Ⅲ (AT-Ⅲ) to inactivate/remove the virus.

S/D method was used as the AT-Ⅲ virus inactivation process in human plasma, and the nanomembrane filtration method (20 nm pore size) was used as the virus removal process. The effect of virus inactivation/removal process on AT-Ⅲ activity was analyzed. Pseudorabies virus, Sindbis virus, porcine parvovirus and encephalomyocarditis virus were used as indicate viruses to verify the virus inactivation/removal effect of the above process.

The method of S/D combined with nanomembrane filtration effectively inactivated/removed lipid-enveloped as well as non-lipid-enveloped viruses, with all indicate viruses titers decreases >4 lg. The biological activity of AT-Ⅲ and other indicators did not change significantly, with activity changes <10%.

S/D combined with nanomembrane filtration method established in this study can effectively inactivate/remove viruses in AT-Ⅲ, providing a reference for the virus inactivation of the coagulation factor products.

Antithrombin-Ⅲ; Virus inactivation/removal; Solvent/detergent treatment method; Nanofiltration method