To investigate the quality stability and influencing factors of mycoplasma detection media stored at 15-25 ℃ for different time.

Four kinds of mycoplasma detection media (mycoplasma broth, mycoplasma semifluid, arginine mycoplasma broth and arginine mycoplasma semifluid) were stored at 15-25 ℃ for 0, 21, 35, 49 and 70 days after sterilization. The appearance, pH and sensitivity of four media were detected at different storage time and the factors causing the change were analyzed.

Three quality indexes of 4 mycoplasma detection media affected each other and changed with the increasing of storage time. During the first 21 days storage period, the internal environmental changes in mycoplasma broth and semiliquid media were the most severe with the most obvious change of color and fast decrease of pH. Three quality indexes of the four mycoplasma media all conformed to the pharmacopoeia standard during the first 35 days storage period, as growing-tube number of corresponding mycoplasma reached at least 2/3 of inoculating-tube number at different dilutions.

At 15-25 ℃, the quality of mycoplasma detection media is qualified during the first 35 days storage period. During the storage period, the sealing of the container determines the amount of air dissolved in the media, and affects the quality stability of media.

Mycoplasma; Culture medium; Quality stability