Effect of actual multiplicity of infection on rotavirus proliferation in large scale production
Shi Chen, Jiang Zhijun, Yang Xinzhe, Ruan Bo, Liu Shengsheng, Liu Yuqing, Zuo Haojie, Tang Yi, Tan Zhen, Bai Xuan, Qiao Jian, Wang Ying, Xu Gelin, Yang Xiaoming
Abstract
ObjectiveTo explore the effect of actual multiplicity of infection(MOI) on virus proliferation in large scale production of rotavirus vaccine.
MethodsThe titer of rotavirus inoculation solution was detected with fluorescent focus assay when inoculated in Vero cells. Actual MOI was calculated as quantity of virus divided by quantity of cells. Cytopathic effect time and single harvest titer were monitored to reflect the effect of actual MOI on rotavirus proliferation in large scale production.
ResultsIn large scale production, MOI in the range of 0.002-0.074 affected the course of cytopathic effect, and showed a weak correlation with the proliferation of rotavirus. The titers of single rotavirus harvest were all above 7.0 lg fluorescent focus unit(FFU)/ml, meeting the quality standard (≥6.5 lgFFU/ml).
ConclusionActual MOI shows a weak correlation with the proliferation of rotavirus in large scale production.
Key words:
Rotavirus vaccines; Vero cells; Multiplicity of infection; Virus proliferation; Large scale production
Contributor Information
Shi Chen
Department of Rotavirus Vaccine, Wuhan Institute of Biological Products Co., Ltd., National Engineering Technology Research Center of Combined Vaccines, Wuhan 430207, China
Jiang Zhijun
Department of Rotavirus Vaccine, Wuhan Institute of Biological Products Co., Ltd., National Engineering Technology Research Center of Combined Vaccines, Wuhan 430207, China
Yang Xinzhe
Department of Rotavirus Vaccine, Wuhan Institute of Biological Products Co., Ltd., National Engineering Technology Research Center of Combined Vaccines, Wuhan 430207, China
Ruan Bo
Department of Rotavirus Vaccine, Wuhan Institute of Biological Products Co., Ltd., National Engineering Technology Research Center of Combined Vaccines, Wuhan 430207, China
Liu Shengsheng
Department of Rotavirus Vaccine, Wuhan Institute of Biological Products Co., Ltd., National Engineering Technology Research Center of Combined Vaccines, Wuhan 430207, China
Liu Yuqing
Department of Rotavirus Vaccine, Wuhan Institute of Biological Products Co., Ltd., National Engineering Technology Research Center of Combined Vaccines, Wuhan 430207, China
Zuo Haojie
Department of Rotavirus Vaccine, Wuhan Institute of Biological Products Co., Ltd., National Engineering Technology Research Center of Combined Vaccines, Wuhan 430207, China
Tang Yi
Department of Rotavirus Vaccine, Wuhan Institute of Biological Products Co., Ltd., National Engineering Technology Research Center of Combined Vaccines, Wuhan 430207, China
Tan Zhen
Department of Rotavirus Vaccine, Wuhan Institute of Biological Products Co., Ltd., National Engineering Technology Research Center of Combined Vaccines, Wuhan 430207, China
Bai Xuan
Department of Rotavirus Vaccine, Wuhan Institute of Biological Products Co., Ltd., National Engineering Technology Research Center of Combined Vaccines, Wuhan 430207, China
Qiao Jian
Department of Rotavirus Vaccine, Wuhan Institute of Biological Products Co., Ltd., National Engineering Technology Research Center of Combined Vaccines, Wuhan 430207, China
Wang Ying
Department of Rotavirus Vaccine, Wuhan Institute of Biological Products Co., Ltd., National Engineering Technology Research Center of Combined Vaccines, Wuhan 430207, China
Xu Gelin
Department of Rotavirus Vaccine, Wuhan Institute of Biological Products Co., Ltd., National Engineering Technology Research Center of Combined Vaccines, Wuhan 430207, China
Yang Xiaoming
Department of Rotavirus Vaccine, Wuhan Institute of Biological Products Co., Ltd., National Engineering Technology Research Center of Combined Vaccines, Wuhan 430207, China
China National Biotec Group Co., Ltd., Beijing 100029, China
