Basic Science Investigation
Automatic synthesis and myocardial imaging of 11C-meta-hydroxyephedrine
He Yulin, Zhang Guojian, Bai Xia, Zhou Weina, Wang Wenrui, Wang Xiangcheng
Published 2022-11-25
Cite as Chin J Nucl Med Mol Imaging, 2022, 42(11): 672-675. DOI: 10.3760/cma.j.cn321828-20210512-00161
Abstract
ObjectiveTo realize a fully automated synthesis of 11C-meta-hydroxyephedrine (mHED) and to perform imaging studies with it.
Methods11C-mHED was prepared by the 11CH3-triflate method. The crude product was purified by semi-preparative high performance liquid chromatograph (HPLC) to obtain the final product. The radiochemical purity and specific activity were determined by radio-HPLC. The myocardial uptake and excretion process of the agent were monitored by microPET/CT imaging on 5 normal SD rats. The clinical imaging value was evaluated using PET/CT imaging in a patient (male, 42 years old) with myocardial infarction.
ResultsThe automated synthesis of 11C-mHED was realized by a commercial synthesizer. The total synthesis time was about 30 min. The radiochemical yield was (15±2)% (non-decay corrected, n=10) and the radiochemical purity was greater than 98%. The specific activity was about 65 GBq/mmol. MicroPET/CT imaging in normal SD rats showed the myocardial uptake was highest at 10 min after the injection of imaging agent, and then the imaging agent was gradually excreted from the myocardium through the liver and gallbladder. PET/CT imaging of a patient with myocardial infarction showed an imaging agent defect near the apex in the inferior wall of the left ventricle, which was matched with results of ultrasound and electrocardiogram examination.
Conclusions11C-mHED can be successfully prepared automatically, with high radiochemical yield and specific activity. It can also highly concentrate in the myocardium, and the imaging effect with this agent is good in a patient with myocardial infarction.
Key words:
Ephedrine; Isotope labeling; Carbon radioisotopes; Positron-emission tomography; Tomography, X-ray computed; Myocardium; Rats
Contributor Information
He Yulin
Department of Nuclear Medicine of the Affiliated Hospital of Inner Mongolia Medical University, Key Laboratory of Molecular Imaging of Inner Mongolia Autonomous Region, Hohhot 010050, China
Zhang Guojian
Department of Nuclear Medicine of the Affiliated Hospital of Inner Mongolia Medical University, Key Laboratory of Molecular Imaging of Inner Mongolia Autonomous Region, Hohhot 010050, China
Bai Xia
Department of Nuclear Medicine of the Affiliated Hospital of Inner Mongolia Medical University, Key Laboratory of Molecular Imaging of Inner Mongolia Autonomous Region, Hohhot 010050, China
Zhou Weina
Department of Nuclear Medicine of the Affiliated Hospital of Inner Mongolia Medical University, Key Laboratory of Molecular Imaging of Inner Mongolia Autonomous Region, Hohhot 010050, China
Wang Wenrui
Department of Nuclear Medicine of the Affiliated Hospital of Inner Mongolia Medical University, Key Laboratory of Molecular Imaging of Inner Mongolia Autonomous Region, Hohhot 010050, China
Wang Xiangcheng
Department of Nuclear Medicine of the Affiliated Hospital of Inner Mongolia Medical University, Key Laboratory of Molecular Imaging of Inner Mongolia Autonomous Region, Hohhot 010050, China
