ORIGINAL ARTICLE
Papanicolaou staining combined with hematoxylin-eosin staining for thyroid liquid-based cytological diagnosis of thyroid lesions
Zhang Yongsheng, Gao Ping, Zhang Liping, Wang Pingping, Han Jie, Li Qiang
Published 2021-01-15
Cite as Chin J Prim Med Pharm, 2021, 28(1): 85-89. DOI: 10.3760/cma.j.issn.1008-6706.2021.01.019
Abstract
ObjectiveTo investigate the application value of papanicolaou staining combined with hematoxylin-eosin staining for thyroid liquid-based cytological diagnosis of thyroid lesions.
MethodsThyroid nodules of 476 patients who received ultrasound-guided fine-needle aspiration cytology from January 2018 to January 2020 in Rizhao Central Hospital were collected for this study. Histopathological diagnosis of these thyroid nodules was obtained after surgery. Two cell smears per specimen were made by liquid-based cytology technology and then stained with papanicolaou and hematoxylin-eosin, respectively. Staining results were divided into three groups according to different staining methods used: papanicolaou,hematoxylin-eosin, papanicolaou + hematoxylin-eosin.After statistical analysis with the Bethesda system for reporting thyroid cytopathology,staining results were compared with histopathological results.
ResultsCytopathological analysis revealed that the detection rate of malignant nodules was 65.5% (312/476) in the papanicolaou group, 64.1% (305/476) in the hematoxylin-eosin group and 74.4% (354/476) in the papanicolaou + hematoxylin-eosin group. The detection rate of malignant nodules in the papanicolaou + hematoxylin-eosin group was significantly higher than that in the other two groups (χ2= 8.816, 11.838, bothP < 0.05). The coincidence rate of thyroid liquid-based cytological diagnosis and postoperative histopathology was 91.2% (434/476) in the papanicolaou group,90.5% (431/476) in the hematoxylin-eosin group and 95.8% (456/476) in the papanicolaou + hematoxylin-eosin group. The coincidence rate of thyroid liquid-based cytological diagnosis and postoperative histopathology in the papanicolaou + hematoxylin-eosin group was significantly higher than that in the other two groups (χ2= 8.350, 10.320, both P < 0.05).
ConclusionThyroid liquid-based cytology with papanicolaou staining combined with hematoxylin-eosin staining can increase the detection rate and diagnostic accuracy of malignant thyroid nodules.
Key words:
Staining and labeling; Biopsy,needle; Histocytological preparation techniques; Chromatin immunoprecipitation; Thyroid diseases; Thyroid nodule; Diagnosis; Pathology,clinical
Contributor Information
Zhang Yongsheng
Department of Pathology, Rizhao Central Hospital, Rizhao 276800, Shandong Province, China
Gao Ping
Department of Pathology, Rizhao Central Hospital, Rizhao 276800, Shandong Province, China
Zhang Liping
Department of Pathology, Rizhao Central Hospital, Rizhao 276800, Shandong Province, China
Wang Pingping
Department of Pathology, Rizhao Central Hospital, Rizhao 276800, Shandong Province, China
Han Jie
Department of Pathology, Rizhao Central Hospital, Rizhao 276800, Shandong Province, China
Li Qiang
Department of Pathology, Rizhao Central Hospital, Rizhao 276800, Shandong Province, China
