Basic Research
Effect of docosapentenoic acid on the neurite outgrowth in PC12 cells
Wang Guanghui, Zhong Ming, Zhang Minna, Wang Tingting, Liang Yuting, Liang Dongrui, Ren Yan, Zhong Chen, Che Jiantu, Wu Jinglong
Published 2017-05-20
Cite as Chin J Behav Med ＆ Brain Sci, 2017,26(05): 390-394. DOI: 10.3760/cma.j.issn.1674-6554.2017.05.002
Abstract
ObjectiveTo explore the inductive action of docosapentenoic acid(DPA) on neurite outgrowth in PC12 cells in vitro.
MethodsNeurite outgrowth in PC12 cells was examined after the treatment with different concentration of DPA using Motic Zamges Plus software mapping cell image system. Western blot was performed to detect the expression of β Ⅲ-tubulin regulated protein kinase, a neuronal marker as well as ERK and protein kinase B (Akt) phosphorylation.
ResultsPC12 cell neurite formation rate was increased in a concentration dependent manner in the induction of DPA, increased by 2.4% (DPA 10 μg/ml, P>0.05), 18.6% (DPA 30 μg/ml, P<0.05) and 25.0% (DPA 50 μg/ml, P<0.05) compared with that in the control group. DPA promoted the expression of β Ⅲ-tubulin (P<0.05) and the phosphorylation level of ERK and Akt (P<0.05, P<0.01).
ConclusionDPA promotes PC12 cell neurites growth and its mechanism may be related to the activation of ERK and Akt signaling pathways.
Key words:
Docosapentenoic acid; PC12 cells; Neurite outgrowth; βⅢ-tubulin; Extracellular regulated protein kinase; Kinase B
Contributor Information
Wang Guanghui
Department of Immune Pharmacology Teaching and Research, College of Pharmacy, Jining Medical University, Rizhao 276826, China
Zhong Ming
Department of Chinese Traditional Medicine Teaching and Research, College of Pharmacy, Jining Medical University, Rizhao 276826, China
Zhang Minna
Department of Pharmaceutical Analysis Teaching and Research, College of Pharmacy, Jining Medical University, Rizhao 276826, China
Wang Tingting
Department of Traditional Chinese Medicine People’s Hospital of Rizhao, Rizhao 276826, China
Liang Yuting
Department of Chinese Traditional Medicine Teaching and Research, College of Pharmacy, Jining Medical University, Rizhao 276826, China
Liang Dongrui
Department of Chinese Traditional Medicine Teaching and Research, College of Pharmacy, Jining Medical University, Rizhao 276826, China
Ren Yan
Department of Immune Pharmacology Teaching and Research, College of Pharmacy, Jining Medical University, Rizhao 276826, China
Zhong Chen
Department of Immune Pharmacology Teaching and Research, College of Pharmacy, Jining Medical University, Rizhao 276826, China
Che Jiantu
S&V Biological Science and Technology Co, Ltd, Beijing 102200, China
Wu Jinglong
Brain Science and Neurotechnology Lab, Beijing Institute of Technology, Beijing 100081, China
