Objective To construct a reliable,effective and practical model for hair follicle reconstitution.Methods Six nude mice were transplanted with four mini-chambers with diameter of 2.5 mm in the dorsal skin respectively.Dermal and epidermal cells were isolated from the skin of newborn C57BL/6 mice.Dermal cells were labeled,then these two kinds of cells were mixed in a ratio of 1 ∶ 1 and injected into the mini-chambers.Hair regeneration was observed grossly,scanning electron microscopically and histologically.Results Four weeks after cell implantation,black hair grew from the skin in six nude mice and 24 transplantation sites.The successful ratio of hair reconstitution was 100％.Newly formed hair follicles were positive for DiI in the dermal papilla and completely developed structure of hair follicle was observed by HE staining.Good-quality hair grew from the skin regularly was observed under the scanning electron microscopy.Conclusion Newborn mice skin cells were transplanted by using mini-chambers,which can construct a model of hair follicle reconstitution successfully.The model has the advantages of small trauma,simple operation and less cells needed,and several transplantation sites can be made,which is a reliable,effective and practical model for hair follicle reconstitution.