Animal Model
Establishment of adjacent disc degeneration model after fixed fusion surgery in New Zealand white rabbits with lumbar disc degeneration
Long Hei, Jianguo Zhao, Liqiong Ma, Fubiao Zhou, Xiucai Ma, Huiqiang Ding
Published 2017-02-08
Cite as Chin J Exp Surg, 2017, 34(2): 342-345. DOI: 10.3760/cma.j.issn.1001-9030.2017.02.052
Abstract
ObjectiveTo establish New Zealand white rabbit model of adjacent disc degeneration after fixed fusion surgery.
MethodsTake 15 male or female New Zealand white rabbits of 10-month-old, with a 18G needle positioning L3-L4 disc under fluoroscopic, and regular observe their magnetic resonance imaging (MRI). We removal of the L3-L4 disc, and take transverse interbody fusion, fix with titanium plate, when L3-L4 intervertebral disc degenerate. Continuously observe they changes of the adjacent disc on MRI after the surgery. We will execute them 12 weeks after surgery, and make histopathologic observation adjacent disc.
ResultsThere is no difference between before and after disc puncturing surgery about the reaction rates of animals(F=1.563, P=0.061). After discectomy and fusion surgery, the reaction rates of animals on different time is different, and the difference is statistically significant (F=6.178, P=0.000). And after discectomy and fusion surgery, all the reaction rates of animals on different time are ireduced compared to pre-operation, and the difference is statistically significant (t=7.952 and 5.791 and 3.944, P=0.000 and 0.000 and 0.000). The survival rate of New Zealand white rabbit is 100%. Continuously observe MRI, we find that the signal of T2 falls in the fourth week after the disc puncturing surgery, and there are different levels degeneration performance of adjacent disc after fusion fixation surgery. The reaction rates of animals on two surgeries is different, and the difference is not statistically significant after 4 weeks (P=0.084). But after 8 or 12 weeks, the difference is statistically significant (P=0.002). When we executed the animals 12 weeks after surgery, we found that the fixed segment is fully integrated and is wrapped around by the poroma. The nucleus of adjacent segment disc is dark colored, and it showed a fat-like changes and the amount is little. HE staining: ill-defined nucleus, nucleus pulposus is replaced by cartilage, central nucleus degenerate.
ConclusionThis research study provides an ideal, reliable animal model for the research of adjacent disc degeneration after discectomy and fusion surgery.
Key words:
New Zealand white rabbits; Disc; Degeneration
Contributor Information
Long Hei
Department of Orthopaedics, the Affiliated Hospital of Ningxia Medical University, Yinchuan 750004, China
Jianguo Zhao
Department of Radiology, the Tumor Affiliated Hospital of Ningxia Medical University, Yinchuan 750004, China
Liqiong Ma
Department of Pathology, the Affiliated Hospital of Ningxia Medical University, Yinchuan 750004, China
Fubiao Zhou
Department of Orthopaedics, the Affiliated Hospital of Ningxia Medical University, Yinchuan 750004, China
Xiucai Ma
Department of Orthopaedics, the Affiliated Hospital of Ningxia Medical University, Yinchuan 750004, China
Huiqiang Ding
Department of Orthopaedics, the Affiliated Hospital of Ningxia Medical University, Yinchuan 750004, China
