The Spindle assembly checkpoint (SAC) is an extremely important monitoring step in the mitosis process, ensuring that all sister chromatids are placed on the medium-term plate before the mid-mitotic transformation. Recent studies have shown that this checkpoint abnormality has a higher correlation with tumorigenesis, development and metastasis mechanisms. Monopolar spindle 1 (Mps1) is a conserved bispecific protein kinase whose primary function is centrosome replication and activation of SAC, both of which help prevent mitotic errors and prevent tumorigenesis. The latest study found that the expression level of Mps1 is related to the degree of malignancy of breast cancer. More notably, inhibition of Mps1 kinase activity or knockdown of Mps1 by siRNA results in a barrier to tumor cell division, leading to tumor cell apoptosis without affecting normal cell activity. The dysregulation of the cell cycle is also closely related to the invasive biological behavior of malignant tumors, and becomes a basic marker of tumor progression. The cell cycle is mainly regulated by a number of checkpoint regulatory pathways, which are specifically dysregulated in tumor cells and Has the potential to become a target for the design of selective treatment of tumor cells. This article reviews how Mps1 regulates SAC and its recent advances in cancer therapy

Spindle assembly checkpoint; Monopolar spindle 1; Gene targeting therapy; Osteosarcoma