张辉; 梁鸿; 白军伟; 王志凯; 郑伟; 秦涛

doi:10.3760/cma.j.issn.1001-9030.2020.03.003

点赞 0
分享 0
收藏 0
纠错

• 胃外科 •

环状RNA circFOXO3在胃癌组织中的表达及其对胃癌细胞增殖和侵袭转移能力的影响

中华实验外科杂志, 2020,37(3) : 408-411. DOI: 10.3760/cma.j.issn.1001-9030.2020.03.003

摘要

目的

观察circFOXO3在胃癌组织的表达及对胃癌细胞增殖和侵袭能力的影响。

方法

收集2017年8月至2018年10月于河南省人民医院胃肠外科进行胃癌根治术的48例胃癌患者组织标本，通过实时定量反转录聚合酶链反应(RT-qPCR)检测circFOXO3在胃癌组织中的表达，分析circFOXO3表达与胃癌组织临床病理特征之间的相关性。构建circFOXO3过表达和对照质粒(pEX-circFOXO3、pEX-NC)、敲减和对照载体(si-circFOXO3、si-NC)，分别设为过表达组和对照组、敲减组和对照组，采用细胞计数(CCK-8)、Transwell侵袭和转移实验检测胃癌细胞的增殖、侵袭和转移能力。计量资料组间比较采用t检验，计数资料采用χ²检验。

结果

circFOXO3在胃癌组织中高表达率(29/48，60.42%)显著高于癌旁正常组织(16/48，33.33%)(χ²＝7.069，P<0.05)，且其表达与组织分化(χ²＝5.976，P<0.05)、浸润深度(χ²＝4.409，P<0.05)、TNM分期(χ²＝5.581，P<0.05)呈正相关。CCK-8、Transwell侵袭和转移实验结果表明，过表达circFOXO3后AGS细胞增殖(24 h：0.95±0.08；48 h：1.76±0.16)、侵袭[(157±11)个]和迁移能力[(186±14)个]均较对照组[24 h：0.58±0.04，t＝7.165，P<0.05；48 h：0.88±0.07，t＝8.728，P<0.05；侵袭为(85±7)个，t＝9.565，P<0.05；迁移为(94±8)个，t＝9.882，P<0.05]显著提高；敲减circFOXO3后SGC-7901细胞增殖(24 h：0.46±0.02，48 h：0.73±0.05)、侵袭[(53±4)个]和迁移[(65±5)个]能力较对照组[24 h：0.63±0.04，t＝6.584，P<0.05；48 h：1.34±0.14，t＝7.107，P<0.05；侵袭为(96±8)个，t＝8.327，P<0.051；迁移为(114±9)个，t＝8.243，P<0.05]均明显降低，差异有统计学意义。

结论

circFOXO3在胃癌组织中显著高表达，且可促进胃癌细胞增殖和侵袭转移。

引用本文: 张辉, 梁鸿, 白军伟, 等. 环状RNA circFOXO3在胃癌组织中的表达及其对胃癌细胞增殖和侵袭转移能力的影响 [J] . 中华实验外科杂志, 2020, 37(3) : 408-411. DOI: 10.3760/cma.j.issn.1001-9030.2020.03.003.

参考文献导出: Endnote NoteExpress RefWorks NoteFirst 医学文献王

扫描看全文

正文

作者信息

基金 0 关键词 0

English Abstract

阅读 0 评论 0

相关资源

引用 | 论文 | 视频

版权归中华医学会所有。

未经授权，不得转载、摘编本刊文章，不得使用本刊的版式设计。

除非特别声明，本刊刊出的所有文章不代表中华医学会和本刊编委会的观点。

环状RNA(circRNA)是一种广泛存在于真核细胞中的非编码RNA，是由RNA剪接产生的一种共价闭合环形RNA分子，主要由外显子和(或)内含子构成^[1]。越来越多的研究表明circRNA在人类肿瘤中发挥重要作用。circFOXO3已被报道在前列腺癌和胶质母细胞瘤中促进肿瘤增殖、抑制凋亡^[2,3]。课题组前期采用全转录组测序发现circFOXO3在胃癌组织中高表达，但其生物学功能和临床意义尚未阐明。本研究旨在探讨circFOXO3在胃癌组织中的表达及其生物学功能。

贡献者信息

张辉

河南省人民医院胃肠外科，郑州　450003

梁鸿

河南省人民医院胃肠外科，郑州　450003

白军伟

河南省人民医院胃肠外科，郑州　450003

王志凯

河南省人民医院胃肠外科，郑州　450003

郑伟

河南省人民医院胃肠外科，郑州　450003

秦涛

河南省人民医院胃肠外科，郑州　450003

通信作者

秦涛

河南省人民医院胃肠外科，郑州　450003

Email：goodfreecn@163.com

关键词

胃癌; circFOXO3; 增殖; 侵袭; 转移;

基金项目

河南省医学科技攻关项目（201303132、2013031636）

利益冲突

所有作者均声明不存在利益冲突

历史

出版日期：2020-03-08

收稿日期：2019-10-13

Stomach Surgery

Expression of circular RNA circFOXO3 in gastric cancer tissues and its effects on proliferation, migration and invasion of gastric cancer cells

Zhang Hui, Liang Hong, Bai Junwei, Wang Zhikai, Zheng Wei, Qin Tao

Published 2020-03-08

Cite as Chin J Exp Surg, 2020, 37(3): 408-411. DOI: 10.3760/cma.j.issn.1001-9030.2020.03.003

Abstract

Objective

To observe the expression of circFOXO3 in gastric cancer tissues and its effects on the proliferation, migration and invasion of gastric cancer cells.

Methods

48 gastric cancer samples were collected from August 2017 to October 2018 in our hospital.The expression of circFOXO3 in gastric cancer tissues was detected by real-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR). The correlations between the expression of circFOXO3 and the clinicopathological characteristics of gastric cancer were analyzed. The overexpression and control plasmids (pEX-circFOXO3, pEX-NC), knock-down and control vectors (si-circFOXO3, si-NC) were constructed, and designed as the overexpression group and control group, knock-down and control group, respectively. Cell counting kit-8 (CCK-8) was used to detect the proliferation of gastric cancer cells, and Transwell migration and invasion assay was used to detect the ability of migration and invasion respectively. The measurement data between groups were compared by T test, and the count data were compared by chi-square test.

Results

The positive expression rate of circFOXO3 in gastric cancer tissues (29/48, 60.42%) was significantly higher than that in adjacent gastric normal tissues (16/48, 33.33%) (χ²= 7.069, P<0.05), and it was positively correlated with the stage of tissue differentiation (χ²= 5.976, P<0.05), infiltration depth (χ²=4.409, P<0.05) and TNM stage (χ²=5.581, P<0.05). The results of CCK-8 and Transwell migration and invasion assays showed that overexpression of circFOXO3 significantly increased the proliferation (24 h: 0.95±0.08; 48 h: 1.76±0.16), invasion [(157±11) cells] and migration [(186±14) cells] ability of AGS cells as compared with the control group [24 h: 0.58±0.04,t=7.165, P<0.05; 48 h: 0.88±0.07,t=8.728, P<0.05; invasion: (85±7) cells,t=9.565, P<0.05; migration: (94±8) cells,t=9.882, P<0.05], while knockdown of circFOXO3 significantly decreased the proliferation (24 h: 0.46±0.02, 48 h: 0.73±0.05), invasion [(53±4) cells] and migration [(65±5) cells] ability of SGC-7901 cells as compared with the control group [24 h: 0.63±0.04,t=6.584, P<0.05; 48 h: 1.34±0.14,t=7.107, P<0.05; invasion: (96±8) cells,t=8.327, P<0.051; migration: (114±9) cells,t=8.243, P<0.05].

Conclusion

CircFOXO3 is highly expressed in gastric cancer tissues, and can promote the proliferation, migration and invasion of gastric cancer cells.

Key words:

Gastric cancer; CircFOXO3; Proliferation; Migration; Invasion

Contributor Information

Zhang Hui