Experimental Study
Identification and analysis of tumor-related gene mutations in 8 human pancreatic cancer cell lines by next-generation sequencing
Wang Qi, Chen Kai, Jin Bo, Yang Yinmo, Tian Xiaodong
Published 2020-04-08
Cite as Chin J Exp Surg, 2020, 37(4): 667-669. DOI: 10.3760/cma.j.cn421213-20200205-00058
Abstract
ObjectiveTo identify and analyze the characteristics of tumor-associated gene mutations in 8 human pancreatic cancer cell lines, in order to provide a basis for the selection of appropriate cell lines in laboratory research of human pancreatic cancer.
MethodsEight human pancreatic cancer cell lines (AsPC-1, BxPC-3, Capan-1, Capan-2, MIA PaCa-2, PANC-1, Patu8988 and T3M4) were purchased from American Type Culture Collection (ATCC) and cultured in vitro, and 113 tumor-associated gene mutations were detected by next-generation sequencing technology. The frequency of mutational genes was calculated, and the functional analysis was performed according to Kyoto Encyclopedia of Genes and Genomes (KEGG) database.
ResultsThe next-generation sequencing showed that mutations in 79 loci of 36 genes were identified in 8 human pancreatic cancer cell lines. The mutation rates of Kirsten ratsarcoma viral oncogene homolog (KRAS), tumor antigen p53 (TP53), cyclin-dependent kinase inhibitor (CDKN2A) and deleted in pancreatic carcinoma locus 4 (DPC4/SMAD4) genes in the 8 cell lines were 87.5% (7/8, except for BxPC-3), 100.0% (8/8), 50.0% (4/8, in AsPC-1, Capan-1, Capan-2 and Patu8988 cells) and 37.5% (3/8, in AsPC-1, Capan-1and T3M4 cells), respectively. Other mutations with higher incidences included AT rich interactive domain 1A (ARID1A) in AsPC-1, Capan-1, Capan-2, MIA PaCa-2 and T3M4 cells, CREB-binding protein gene (CREBBP) in Capan-1, Capan-2, MIA PaCa-2 and T3M4 cells, NOTCH1 in BxPC-3, Capan-2, MIA PaCa-2and T3M4, adenomatous polyposis coli (APC) in AsPC-1, Capan-1 and Patu8988, and E1A binding protein p300 (EP300) in BxPC-3, MIA PaCa-2 and T3M4. The functions of the 36 mutated genes were complex, involving Ras/MAPK signaling pathway, cell cycle transition, Wnt/β-catenin and NOTCH signaling pathway, chromatin remodeling complex family, TGF-β signaling pathway, DNA damage repair, Hedgehog and PI3K-Akt signaling pathways.
ConclusionThe 8 human pancreatic cancer cell lines are genetically representative of human pancreatic cancer tissues. Different cell lines have different characteristics of gene mutations, and the laboratory research in human pancreatic cancer should use appropriate cell lines according to their genetic characteristics.
Key words:
Pancreatic cancer; Cell line; Gene mutation; Next-generation sequencing
Contributor Information
Wang Qi
Department of General Surgery, Peking University First Hospital, Beijing 100034, China
Chen Kai
Department of General Surgery, Peking University First Hospital, Beijing 100034, China
Jin Bo
Department of Clinical Laboratory, Peking University First Hospital, Beijing 100034, China
Yang Yinmo
Department of General Surgery, Peking University First Hospital, Beijing 100034, China
Tian Xiaodong
Department of General Surgery, Peking University First Hospital, Beijing 100034, China
