Clinical Research
Identification of hub genes as prognostic biomarkers for triple-negative breast cancer using integrating bioinformatics analysis
Yang Lijie, Yang Tiecheng, Shi Liwen, Wang Huaqiao, Wang Danwen, Li Xuanfei, Xie Wei
Published 2020-08-08
Cite as Chin J Exp Surg, 2020, 37(8): 1505-1508. DOI: 10.3760/cma.j.cn421213-20200224-01073
Abstract
ObjectiveThis study aims to search for differentially expressed genes (DEGs), which may be involved in the pathogenesis of triple-negative breast cancer (TNBC) through analysis of gene expression profiles of TNBC compared with non-TNBC.
MethodsFour mRNA microarray datasets from gene expression omnibus (GEO) were downloaded and analyzed to obtain DEGs. The protein-protein interaction (PPI) network of DEGs was constructed, and hub genes were filtered by STRING and Cytoscape. Bioinformatics analysis was used for functional enrichment analyses, including gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG). UCSC, ClueGO, Oncomine, and Kaplan-Meier plotter online websites are used to analyze expression, and survival levels of hub genes.
ResultsA total of 287 DEGs and 10 hub genes were recognized between TNBC and non-TNBC. Kaplan-Meier survival curves indicated that higher expression of epidermal growth factor receptor (EGFR) or lower expression of ESR1 and insulin-like growth factor (IGF)-1 were correlated with poorer prognosis in TNBC patients. Patients with non-TNBC exhibited prognosis characteristics significantly differing from those with TNBC.
ConclusionESR1, EGFR and IGF-1 might have been involved in poor prognosis and recurrence of TNBC.
Key words:
Triple-negative breast cancer; Differentially expressed gene; Biomarker; Prognosis
Contributor Information
Yang Lijie
Department of Gastrointestinal Surgery, Zhongnan Hospital of Wuhan University, Clinical Medical Research Center of Peritoneal Cancer of Wuhan, Clinical Cancer Study Center of Hubei Province, Key Laboratory of Tumor Biological Behavior of Hubei Province, Wuhan 430071, China
Yang Tiecheng
Department of Gastrointestinal Surgery, Zhongnan Hospital of Wuhan University, Clinical Medical Research Center of Peritoneal Cancer of Wuhan, Clinical Cancer Study Center of Hubei Province, Key Laboratory of Tumor Biological Behavior of Hubei Province, Wuhan 430071, China
Shi Liwen
Department of Gastrointestinal Surgery, Zhongnan Hospital of Wuhan University, Clinical Medical Research Center of Peritoneal Cancer of Wuhan, Clinical Cancer Study Center of Hubei Province, Key Laboratory of Tumor Biological Behavior of Hubei Province, Wuhan 430071, China
Wang Huaqiao
Department of Gastrointestinal Surgery, Zhongnan Hospital of Wuhan University, Clinical Medical Research Center of Peritoneal Cancer of Wuhan, Clinical Cancer Study Center of Hubei Province, Key Laboratory of Tumor Biological Behavior of Hubei Province, Wuhan 430071, China
Wang Danwen
Department of Gastrointestinal Surgery, Zhongnan Hospital of Wuhan University, Clinical Medical Research Center of Peritoneal Cancer of Wuhan, Clinical Cancer Study Center of Hubei Province, Key Laboratory of Tumor Biological Behavior of Hubei Province, Wuhan 430071, China
Li Xuanfei
Department of Gastrointestinal Surgery, Zhongnan Hospital of Wuhan University, Clinical Medical Research Center of Peritoneal Cancer of Wuhan, Clinical Cancer Study Center of Hubei Province, Key Laboratory of Tumor Biological Behavior of Hubei Province, Wuhan 430071, China
Xie Wei
Department of Gastrointestinal Surgery, Zhongnan Hospital of Wuhan University, Clinical Medical Research Center of Peritoneal Cancer of Wuhan, Clinical Cancer Study Center of Hubei Province, Key Laboratory of Tumor Biological Behavior of Hubei Province, Wuhan 430071, China
