Clinical Research
The relationship between microRNA-150 expression and epithelial-mesenchymal transition in gallbladder cancer and its mechanism
Li Tao, Ma Lifeng, Liu Guochao, Wang Jianlong, Yin Zhaoqiang, Zhang Zhanxue
Published 2020-10-08
Cite as Chin J Exp Surg, 2020, 37(10): 1890-1893. DOI: 10.3760/cma.j.cn421213-20200108-01017
Abstract
ObjectiveTo analyze the relationship between the expression of microRNA (miRNA, miR)-150 and epithelial-mesenchymal transition (EMT) in gallbladder cancer and its mechanism.
MethodsThe expression of miR-150 in 30 cases of gallbladder cancer was detected by real-time quantitative polymerase chain reaction (qPCR), and 30 cases of chronic cholecystitis tissues was used as control. Simultaneously, the expression of proliferating cell nuclear antigen (PCNA), E-cadherin, N-cadherin and matrix metalloproteinase (MMP)-9 was detected, and the relationship of miR-150 and these genes was analyzed. Human gallbladder cancer cell line GBC-SD was transfected with miR-150 mimics. Then scratch experiments and Transwell chamber experiments were used to detect changes in cell migration and invasion; qPCR and Western blotting were used to detect changes in mRNA and protein of each gene.
ResultsThe expressions of miR-150 and E-cadherin in gallbladder cancer tissues were lower than those in chronic cholecystitis tissues (t=-7.035, -14.146, P<0.01). The expressions of PCNA, N-cadherin and MMP-9 were significantly higher compared with chronic cholecystitis tissues (t=3.813, 9.339, 5.616, P<0.01). The expression of miR-150 was related to the degree of tumor differentiation, clinical stage, and lymph node metastasis (t=3.228, 2.396, -2.604, P<0.05). Pearson correlation analysis showed that miR-150 was positively correlated with E-cadherin (r=0.630, P<0.05), and negatively correlated with PCNA, N-cadherin, and MMP-9 (r=-0.769, -0.628, -0.616, P<0.05). After transfection, the migration and invasion ability of GBC-SD cells decreased (F=1 965.860, 114.940, P<0.01). The expressions of PCNA, N-cadherin, and MMP-9 were decreased, while the expression of E-cadherin was increased (P<0.05).
ConclusionDecreased miR-150 expression in gallbladder cancer plays a key role in tumor progression, and the mechanism may be that miR-150 participates in tumor EMT process by regulating some genes.
Key words:
Gallbladder cancer; MicroRNA-150; Epithelial-mesenchymal transition; Clinicopathological parameters; Experiments in vitro
Contributor Information
Li Tao
Department of General Surgery, the Second Hospital of Hebei Medical University, Shijiazhuang 050061, China
Ma Lifeng
Department of General Surgery, the Second Hospital of Hebei Medical University, Shijiazhuang 050061, China
Liu Guochao
Department of General Surgery, the Second Hospital of Hebei Medical University, Shijiazhuang 050061, China
Wang Jianlong
Department of General Surgery, the Second Hospital of Hebei Medical University, Shijiazhuang 050061, China
Yin Zhaoqiang
Department of General Surgery, the Second Hospital of Hebei Medical University, Shijiazhuang 050061, China
Zhang Zhanxue
Department of General Surgery, the Second Hospital of Hebei Medical University, Shijiazhuang 050061, China
