Experimental Study
Osteogenic effect of exosomes derived by bEnd.3 cells: an in vitro study
Cui Yongzhi, Liu Ping, Liang Tao, Li Rui, Geng Yongtao, Liu Huimin, Fu Dehao
Published 2021-03-08
Cite as Chin J Exp Surg, 2021, 38(3): 507-510. DOI: 10.3760/cma.j.cn421213-20200727-01267
Abstract
ObjectiveTo investigate the osteogenic effect of exosomes derived by mouse brain microvascular endothelial cell line bEnd.3 on bone marrow mesenchymal stem cells (BMSCs).
MethodsUltracentrifuge was used to extract the exosomes derived by brain-derived endothelial cells.3 (bEnd.3) cells bEnd.3 cells derived exosomes (b-Exo). Transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA) and Western blotting were use to characterize the exosomes. Alizarin red staining was used to detect the osteogenic effects of the exosomes. The relative messenger ribonucleic acid (mRNA) expression of osteogenic-related genes runt-related transcription factor 2 (Runx2), osteopontin and osteocalcin was detected by reverse transcriptase-polymerase chain reaction (RT-PCR).
ResultsTEM showed that the particles presented a typical vesicle-like shape. NTA manifested the concentration of Exo was 7.5×1010 particles/ml and the diameter was (100.0±7.3) nm. The b-Exo could enhance the BMSCs matrix mineralization and increase the mRNA expression of osteogenic-related genes Runx2, osteopontin and osteocalcin compared to the controls (8.28±0.34 vs. 3.71±0.40, t=4.974, P<0.01; 9.19±1.91 vs. 4.09±0.88,t=5.562, P<0.01; 10.31±1.67 vs. 5.32±0.31,t=5.432, P<0.01).
ConclusionThe b-Exo could promote the osteogenic performance of BMSCs and may be applied to the bone regeneration and the cell-free treatment of osteoporosis in the near future.
Key words:
Exosomes; Osteoporosis; Bone marrow mesenchymal stem cells; Endothelial cells; Osteogenesis
Contributor Information
Cui Yongzhi
Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
Liu Ping
Physical Examination Center, Liyuan Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430077, China
Liang Tao
Department of Laboratory Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
Li Rui
Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
Geng Yongtao
Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
Liu Huimin
Department of Internal Emergency Medicine, Liyuan Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430077, China
Fu Dehao
Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
