Cardio-thoracic Surgery
The role of microRNA-22 in the occurrence and development of aortic valve calcification by inhibiting the expression of sirtuin 6
Xie Xiaoyong, Feng Xu, He Zhou, Zhu Xiaoli, Weng Dashuai
Published 2022-08-08
Cite as Chin J Exp Surg, 2022, 39(8): 1476-1479. DOI: 10.3760/cma.j.cn421213-20211111-01323
Abstract
ObjectiveTo investigate the effect of microRNA-22 (miR-22) on the osteogenic differentiation of valve interstitial cells and its role in the occurrence and development of aortic valve calcification.
MethodsTotally, 15 patients with calcified aortic valve disease resected during aortic valve replacement were selected, of which calcified aortic valve tissue was used as the experimental group and normal aortic valve tissue as the control group. Hematoxylin and eosin (HE) staining and VonKossa method were used to observe the degree of valve calcification. Real-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR) and immunofluorescence assays were used to detect the expression changes and protein expression levels of miR-22 in valve tissue.
ResultsHE staining and Von Kossa calcium deposition staining suggested that there was a large amount of hydroxyapatite deposition in the experimental group. The immunohistochemical staining results showed positive expression of Runx2 and OCN in the experimental group. The miR-22 gene expression in the experimental group was significantly higher than in the control group (P<0.01). Runx2, a bone related gene in the experimental group, was higher than that in the control group (1.39±0.02 vs. 1.09±0.01, F=4.00, P<0.01). The expression of the bone related gene OCN was higher in the experimental group than in the control group (0.92±0.03 vs. 0.58±0.02, F=2.250, P<0.01), and histone deacetylase 6 (HDAC6) was lower in the experimental group than in the control group (1.09±0.05 vs. 1.52±0.04, F=1.563, P<0.01). The results of dual luciferin reporter assay and Western blotting assay showed that miR-22 overexpression could significantly increase the expression of osteogenesis related genes. Overexpression of miR-22 significantly attenuated HDAC6 expression.
ConclusionMiR-22 promotes the expression and activity of Runx2 and OCN by inhibiting the expression of HDAC6, and the osteogenic differentiation of valve interstitial cells is involved in the process of aortic valve calcification.
Key words:
MicroRNA-22; Aortic valve calcification; Sirtuin 6; Gene expression
Contributor Information
Xie Xiaoyong
Department of Cardiac Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning 530000, China
Feng Xu
Department of Cardiac Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning 530000, China
He Zhou
Department of Cardiac Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning 530000, China
Zhu Xiaoli
Department of Cardiac Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning 530000, China
Weng Dashuai
Department of Cardiac Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning 530000, China
