Objective To study imaging through 18 F-FDG PET-CT on established rabbit tumor model of VX2. Methods VX2 tumor cells were routinely cultured. VX2 cells were diluted to the concentration to 107/ml with RPMII640, and 1.0 ml was injected into neck skin of rabbits. Rabbits were inspected by 18 F-FDG PET-CT every week. After last inspection, rabbits were sacrificed. Tumor tissues were taken out and pathological sections were stained with HE. Results Tumor was found in 4 of 10 rabbits. In the first week, there was no abnormal radioactive accumulation. Since the second week, radioactive accumulation were found in 4 rabbits, and the mean diameter was 0. 8em ×0. 6cm. After three weeks, the average tumor size was 1.8cm × 1.5 cm. CT showed uniform density inside the tumor, CT value was about 30HU. PET showed obvious high radio-absorbance, T/M value was about 9. 2. Conclusion A rabbit tumor model was constructed through subcutaneous injection of VX2 tumor cells and the ratio of tumor formed was 40%. Imaging of clinical PET-CT demonstrated that VX2 tumor tissues could uptake 18 F-FDG more than normal tissue, which made the basis for further study of VX2 tumor model.