Monographic Research·Clinical Management of Acute Respiratory Infectious Diseases
Study on the mechanism of Danpi-Chishao in the treatment of sepsis based on network pharmacology
Su Jiahui, Wu Caijun, Nan Fuyao, Xia Huan, Ren Yang, Ma Linqin
Published 2023-02-20
Cite as J Chin Physician, 2023, 25(2): 178-185. DOI: 10.3760/cma.j.cn431274-20230204-00093
Abstract
ObjectiveTo analyze the mechanism of Danpi-Chishao in treatment of sepsis based on network pharmacology.
MethodsThe corresponding targets of Danpi-Chishao and sepsis were carried out through TCMSP database, OMIM database and Genecards database. Cystoscope 3.8.2 software was used to construct the " Chinese medicine-active components-target-disease" network diagram. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were carried out by DAVID database. Weisheng cloud platform was used to draw bubble map.
ResultsA total of 36 effective components of Danpi-Chishao was obtained, mainly including quercetin, kaempferol, baicalin, β-sitosterol, stigmasterol, paeoniflorin and so on. There were 96 potential common key targets between Danpi-Chishao and sepsis, such as prostaglandin-endoperoxide synthase 2 (PTGS2), transcription factor p65 (RELA), phosphatidylinositol-4, 5-bisphosphate 3-kinase catalytic subunit gamma (PIK3CG), B-cell lymphoma 2 (BCL-2)-associated X (BAX), BCL-2, Caspase-3 (CASP3) with a degree value>4.9. The result of protein-protein interaction (PPI) network analysis showed that there were 10 important target proteins, including alpha serine/threonine-protein kinase (AKT1), interleukin-6 (IL-6), tumor necrosis factor (TNF), interleukin-1β (IL-1β), vascular endothelial growth factor A (VEGFA), cellular tumor antigen p53 (TP53), matrix metalloproteinase-9 (MMP9), CASP3, PTGS2, C-C motif chemokine ligand 2 (CCL2). The pathways obtained by GO and KEGG enrichment analysis included atherosclerosis pathway, advanced glycation end products (AGE)-receptor for advanced glycation end products (RAGE) signal pathway, cancer pathway, tumor necrosis factor signal pathway, hypoxia-inducible factor (HIF) signal pathway, IL-17 signal pathway and other pathway.
ConclusionsThe mechanism of the intervention effect of Danpi-Chishao on sepsis may be that the active components such as quercetin, kaempferol, paeoniflorin act on target proteins such as PTGS2, RELA, PIK3CG, BAX, BCL2, CASP3, and through TNF-related signal pathway, HIF-1 signal pathway, IL-17 signal pathway, etc. Nonetheless, the conclusion needs further experimental verification.
Key words:
Sepsis; Paeoniae radix rubra; Moutan cortex; Network pharmacology
Contributor Information
Su Jiahui
Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
Beijing University of Chinese Medicine, Beijing 100105, China
Wu Caijun
Department of Emergency, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
Institute of Sepsis, Beijing University of Chinese Medicine, Beijing 100700, China
Nan Fuyao
Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
Beijing University of Chinese Medicine, Beijing 100105, China
Xia Huan
Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
Ren Yang
Department of Emergency, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
Institute of Sepsis, Beijing University of Chinese Medicine, Beijing 100700, China
Ma Linqin
Department of Emergency, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
Institute of Sepsis, Beijing University of Chinese Medicine, Beijing 100700, China
