Objective To understand the drug resistance of ureaplasma urealyticum (UU) and mycoplasma hominis (MH),in order to provide scientific basis for the rational use of antibiotics from April 2010 to April 2011 among non-gonococcal urethritis (NGU) patients in this area.Methods The mycoplasma culture and drug sensitivity test were done using kit produced by Zhuhai Lizhu Co.,Ltd.The UU and MH infection levels and the result s of drug sensitivity test were analyzed.Results There were 2622 cases found mycoplasma positive,with a total detection rate of 28.7％,including 2300 strains of UU (25.2％),274 strains of UU + MH (3.0 ％)and 48 strains of MH (1.0％).The susceptibility rates of the 9 kinds of antibiotics:minocycline,levofloxacin,ofloxacin,sparfloxacin,clarithromycin,azithromycin,roxithromycin,josamycin and doxycycline.The drug sensitivity test of 9 antibiotics showed that the sensitivity rates to UU:92.0％,13.9％,19.1％,27.4％,62.4％,30.4％,14.7％,89.4％,90.9％,MH:100.0％,50.0％,42.3％,76.9％,3.8％,0.0％,0.0％,100.0％,100.0％,UU + MH:92.0％,13.9％,9.1％,27.4％,2.6％,2.2％,1.8％,89.4％,90.9％.Pure strains of UU sensitivity to antibiotics was followed by josamycin,minocycline doxycycline,clarithromycin.Mixed infection of strain sensitivity to antibiotics was followed by josamycin,minocycline,doxycycline.MH to antibiotics was followed by minocycline,josamycin,doxycycline,.Conclusion Mcoplasma is one of the major pathogens of sexually transmited disease.Sensitivity test showed that most strains are resistant to mixed infections more severe drug resistance,suggesting that the clinical use of antibiotics should be reasonable.The urinary tract infections to prevent the increase in drug-resistant strains in order to improve the curative effect.The major pathogeny for NGU patients are (UU and MH),minocycline,doxycycline,josamycin will be the first choice,inadvisable choice roxithromycin,azithromycin,clarithromycin for NGU patients in this area.