Objective To compare the clinical efficacy of tobramycin and commonly used drug in the treatment of bacterial infection in pediatric eye disease,and to analyze security of tobramycin in children with bacterial infection eye diseases.Methods From March 2011 to March 2013 155 children with bacterial infection eye diseases in our hospital were selected as object of study and randomly divided into experimental group and control group.Experimental group was treated with tobramycin,while control group was commonly treated with ofloxacin.Compared the efficacy,bacterial sensitivity,bacterial clearance rate and adverse reactions of two groups after treatment.Results The clinical total effective rate in experimental group was 93.3％,that in control group was 75.0％,with significant difference between two groups (P ＜ 0.05).The bacterial sensitivity of tobramycin was 92.0％,that of ofloxacin was 90.0％,without significant difference (P ＞ 0.05).The bacterial clearance rate of tobramvcin was 95.2％,that of ofloxacin was 73.5％,with significant difference (P ＜ 0.05).There was l case of ocular irritation pain in experimental group,and 15 cases in control group.Conclusion Tobramycin in the treatment of bacterial infection in children with eye disease is more effective than ofloxacin,with high security,worthy of clinical application.