Treatises
Pregnancy outcomes of intracytoplasmic sperm injection with sperm from different sources
Huang Cuiyu, Wang Lili, Wang Fang, Zhu Zhaoping, Xiao Zonghui, Huang Ju
Published 2020-01-15
Cite as IMHGN, 2020, 26(2): 198-202. DOI: 10.3760/cma.j.issn.1007-1245.2020.02.015
Abstract
ObjectiveTo compare the pregnancy outcomes after intracytoplasmic sperm injection (ICSI) of three different sources of spermatozoa in the previous conventional In vitro fertilization IVF) cycle, including ejaculated spermatozoa from patients with severe oligoasthenozoospermia and those with testicular spermatozoa and from patients with obstructive azoospermia.
MethodsSperms were obtained by ejaculation of patients with non- or low-fertilization (group A, n=45) and those with severe oligoasthenospermia (group B, n=345) in the previous routine IVF cycle. Sperm were obtained by testicular aspiration (TESA) from patients with obstructive azoospermia (group C, n=110). The fertilization, pregnancy, and implantation rates after ICSI were compared.
ResultsThe age of the woman and the number of years of infertility in group A were significantly higher than those in groups B and C (all P<0.05). The fertilization rate and cleavage rate in group C were significantly lower than those in groups A and B (allP<0.05). The fertilization rate of 2PN (2 pronucleus) among the three groups was statistically different, and the fertilization rate of 2PN in group B was the highest (72.11%, 73.85%, 63.02%). There were no statistical differences between the three groups in the high quality embryo rate (61.50%, 66.09%, 67.96%) and available blastocyst rate (25.77%, 26.27%, 27.67%) (allP>0.05). The clinical pregnancy rate (68.42%, 71.97%, 69.89%), embryo implantation rate (49.30%, 50.17%, 45.78%), delivery rate (55.26%, 60.83%, 53.76%), single pregnancy rate (42.86%, 52.36%, 68.00%), multiple pregnancy rate (57.14%, 47.64%, 32.00%), ectopic pregnancy rate (0.00%, 0.88%, 1.54%), abortion rate (7.69%, 8.41%, 12.31%) and premature delivery rate (33.33%, 28.80%, 26.00%) were not statistically different between the 3 groups (allP>0.05).
ConclusionICSI performed by sperm from different sources has an impact on the fertilization rate of oocytes. group B had the highest normal fertilization rate, and group C had the lowest fertilization rate and cleavage rate, suggesting that testicular sperm has a negative impact on the fertilization rate and early embryo development, but all three groups could achieve good pregnancy outcomes.
Key words:
Intracytoplasmic sperm injection; Azoospermia; Oligospermia; Asthenospermia; Abnormal sperm disease; Testis; Pregnancy outcomes
Contributor Information
Huang Cuiyu
Guangdong Maternal and Child Health Hospital, Guangzhou 51010, China
Wang Lili
Heyuan Maternal and Child Health Hospital, Heyuan 517000, China
Wang Fang
Guangdong Maternal and Child Health Hospital, Guangzhou 51010, China
Zhu Zhaoping
Guangdong Maternal and Child Health Hospital, Guangzhou 51010, China
Xiao Zonghui
Guangdong Maternal and Child Health Hospital, Guangzhou 51010, China
Huang Ju
Guangdong Maternal and Child Health Hospital, Guangzhou 51010, China